Nitrosylation of rabbit ferrous heme-hemopexin

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Mauro FasanoPaolo Ascenzi

Abstract

Hemopexin (HPX) serves as a trap for toxic plasma heme, ensuring its complete clearance by transportation to the liver. Moreover, HPX-heme has been postulated to play a key role in the homeostasis of nitric oxide (NO). Here, the thermodynamics for NO binding to rabbit ferrous HPX-heme as well as the EPR and optical absorption spectroscopic properties of rabbit ferrous nitrosylated HPX-heme (HPX-heme-NO) are reported. The value of the dissociation equilibrium constant for NO binding to rabbit ferrous HPX-heme (i.e., H) is (1.4+/-0.2)x10(-7) M, at pH 7.0 and 10.0 degrees C; the value of H is unaffected by sodium chloride. At pH 7.0, rabbit ferrous HPX-heme-NO is a six-coordinate heme-iron species, characterized by an X-band EPR spectrum with an axial geometry and by epsilon=146 mM(-1) cm(-1) at 419 nm. At pH 4.0, rabbit ferrous HPX-heme-NO is a five-coordinate heme-iron species, characterized by an X-band EPR spectrum with three-line splitting centered at 334 mT and by epsilon=74 mM(-1) cm(-1) at 387 nm. The p K(a) value of the reversible pH-induced six- to five-coordinate spectroscopic transition is 4.8+/-0.1 in the absence of sodium chloride and 4.3+/-0.1 in the presence of 1.5x10(-1) M sodium chloride. This result is in agreem...Continue Reading

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Citations

Sep 8, 2009·The Journal of Biological Chemistry·Paolo AscenziMauro Fasano
Jun 30, 2009·Biochemical and Biophysical Research Communications·Paolo AscenziMauro Fasano
Dec 26, 2006·Biochemical and Biophysical Research Communications·Paolo Ascenzi, Mauro Fasano
Jan 19, 2007·The FEBS Journal·Paolo AscenziMauro Fasano
May 12, 2010·The FEBS Journal·Paolo AscenziMassimo Coletta
May 16, 2006·Biochemical and Biophysical Research Communications·Mauro FasanoPaolo Ascenzi
Dec 25, 2012·Biochemical and Biophysical Research Communications·Paolo AscenziSylvia Dewilde
Nov 6, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Mar 4, 2006·IUBMB Life·Paolo AscenziMauro Fasano
Apr 19, 2005·Journal of Inorganic Biochemistry·Paolo AscenziMassimo Coletta

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