Nitrotyrosine proteome survey in asthma identifies oxidative mechanism of catalase inactivation.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Sudakshina GhoshSerpil C Erzurum

Abstract

Reactive oxygen species and reactive nitrogen species produced by epithelial and inflammatory cells are key mediators of the chronic airway inflammation of asthma. Detection of 3-nitrotyrosine in the asthmatic lung confirms the presence of increased reactive oxygen and nitrogen species, but the lack of identification of modified proteins has hindered an understanding of the potential mechanistic contributions of nitration/oxidation to airway inflammation. In this study, we applied a proteomic approach, using nitrotyrosine as a marker, to evaluate the oxidation of proteins in the allergen-induced murine model of asthma. Over 30 different proteins were targets of nitration following allergen challenge, including the antioxidant enzyme catalase. Oxidative modification and loss of catalase enzyme function were seen in this model. Subsequent investigation of human bronchoalveolar lavage fluid revealed that catalase activity was reduced in asthma by up to 50% relative to healthy controls. Analysis of catalase isolated from asthmatic airway epithelial cells revealed increased amounts of several protein oxidation markers, including chloro- and nitrotyrosine, linking oxidative modification to the reduced activity in vivo. Parallel in vi...Continue Reading

References

Sep 1, 1977·The Journal of Experimental Medicine·J T KungD W Talmage
Jun 1, 1979·British Journal of Cancer·G A CurrieL Cifuentes
Mar 1, 1992·The American Review of Respiratory Disease·W J CalhounW W Busse
Sep 30, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·Z NovákB Matkovics
Jan 1, 1989·International Archives of Allergy and Applied Immunology·G J GleichS J Ackerman
Jan 1, 1984·Methods in Enzymology·H Aebi
Dec 18, 1993·Lancet·Q HamidJ M Polak
Oct 1, 1993·American Journal of Respiratory Cell and Molecular Biology·L KobzikJ S Stamler
Jun 1, 1993·The Journal of Clinical Investigation·J W HeineckeJ A Goldstein
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L A MacMillan-CrowJ A Thompson
Nov 1, 1996·The American Journal of Physiology·J S Beckman, W H Koppenol
Apr 16, 1998·Protein Science : a Publication of the Protein Society·A DucretR Aebersold
Jul 31, 1998·Archives of Biochemistry and Biophysics·H Ischiropoulos
Nov 10, 1998·The Biochemical Journal·G Wu, S M Morris
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·S A ComhairS C Erzurum
Nov 5, 1999·Archives of Biochemistry and Biophysics·J M SouzaH Ischiropoulos
May 17, 2000·The Journal of Clinical Investigation·W WuS L Hazen
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F H GuoS C Erzurum
Aug 18, 2000·Free Radical Biology & Medicine·E A PodrezS L Hazen
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A ComhairS C Erzurum
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A DweikS C Erzurum
Apr 18, 2001·American Journal of Respiratory Cell and Molecular Biology·R A DweikS C Erzurum
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J C MacPhersonS L Hazen
Apr 24, 2001·American Journal of Respiratory and Critical Care Medicine·H IijimaD H Eidelman
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J P GautJ W Heinecke
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·K S AulakD J Stuehr
Oct 12, 2001·Archives of Biochemistry and Biophysics·P S WongA van der Vliet
Oct 24, 2001·American Journal of Respiratory and Critical Care Medicine·A DuguetD H Eidelman
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·S B KhatriS C Erzurum
Jun 11, 2002·Annual Review of Nutrition·Sidney M Morris
Jun 19, 2002·Protein Science : a Publication of the Protein Society·Jana SopkovaJacques Gallay

❮ Previous
Next ❯

Citations

May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kewal AsosinghSerpil C Erzurum
Sep 25, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kei TakemotoHironobu Ishiyama
Nov 1, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J G WagnerD B Peden
Feb 20, 2008·Expert Review of Proteomics·Farrah Kheradmand, David B Corry
Jan 29, 2010·The Journal of Biological Chemistry·Weifeng SongSadis Matalon
Oct 1, 2007·Expert Review of Respiratory Medicine·Stewart J Levine
Jul 5, 2011·European Journal of Clinical Investigation·Elisabeth FabianIbrahim Elmadfa
May 17, 2011·PloS One·Suzy A A ComhairUNKNOWN National Heart Lung Blood Institute Severe Asthma Research Program
Dec 10, 2013·Mass Spectrometry Reviews·Xianquan ZhanDominic M Desiderio
Oct 6, 2020·Oxidative Medicine and Cellular Longevity·Patrick C BradshawWilliam M Curtis
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karina CklessYvonne M W Janssen-Heininger
Oct 31, 2008·Proteomics·Ingrid MillerElisabetta Gianazza
Aug 15, 2009·Journal of Cellular Physiology·Andy T Y Lau, Jen-Fu Chiu
May 15, 2007·Immunological Reviews·Emily J Swindle, Dean D Metcalfe
Aug 5, 2010·Bioelectromagnetics·Roza K SypniewskaFrank A Witzmann
Jan 15, 2013·Pediatric Research·Alexandra SittkaBernd T Schmeck
Dec 1, 2013·Annals of the American Thoracic Society·Fernando Holguin
Aug 8, 2012·Antioxidants & Redox Signaling·Sudakshina GhoshSerpil C Erzurum
May 3, 2014·Journal of Toxicology and Environmental Health. Part a·Renate HochscheidBernd Müller
Dec 6, 2017·International Journal of Molecular Sciences·Michela Bullone, Jean-Pierre Lavoie
Jul 29, 2009·Antioxidants & Redox Signaling·Suzy A A Comhair, Serpil C Erzurum
Nov 13, 2010·Folia Histochemica Et Cytobiologica·Barbara KamerHenryk Tchórzewski
Nov 19, 2008·Antioxidants & Redox Signaling·M Carmen Martínez, Ramaroson Andriantsitohaina
Jul 19, 2008·American Journal of Respiratory and Critical Care Medicine·Abigail LaraUNKNOWN National Heart, Lung, and Blood Institute's Severe Asthma Research Program
Dec 31, 2010·Respiratory Research·Nao OdajimaMasaharu Nishimura
May 1, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Usha RajMichael A Matthay
Feb 1, 2008·Expert Review of Respiratory Medicine·Andrew J Ghio
Sep 10, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A V PolonikovM A Solodilova
May 24, 2016·The Journal of Clinical Investigation·Weiling XuSerpil C Erzurum
Feb 7, 2012·Antioxidants & Redox Signaling·Anne M FitzpatrickLou Ann S Brown
Aug 15, 2019·The Journal of Biological Chemistry·Matías N MöllerBeatriz Alvarez
Jul 31, 2019·International Journal of Molecular Sciences·Celien LismontMarc Fransen
Nov 30, 2006·Redox Report : Communications in Free Radical Research·Tiffany A Reiter
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Apr 20, 2011·Expert Review of Proteomics·Choon-Sik Park, Taiyoun Rhim
Jan 20, 2009·American Journal of Respiratory Cell and Molecular Biology·Yashoda M HosakoteAntonella Casola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.