PMID: 2507453Oct 15, 1989Paper

NK sensitivity and lung clearance of MHC-class-I-deficient cells within a heterogeneous fibrosarcoma

International Journal of Cancer. Journal International Du Cancer
I AlgarraK Kärre

Abstract

The present study examines clonal variations in NK sensitivity in a methylcholanthrene-induced fibrosarcoma. Previous studies of clones from this tumor have shown considerable heterogeneity in H-2 expression, and an association between deleted or low levels of class-I products and increased tumorigenicity after subcutaneous implantation in immunocompetent syngeneic mice. Here, fibrosarcoma clones with no or low expression of MHC-class-I products were found to be sensitive to NK-mediated lysis, while clones with high levels of MHC-class-I expression were relatively resistant. One H-2+ (G2) and one H-2- (B9) clone were chosen for more detailed studies. Cold-target competition assays and conjugate cytotoxicity assays in agarose showed that splenic effector cells bound equally well to the H-2+ and H-2- tumor clone, although only the latter was sensitive to NK cell lysis. Treatment with 50 U/ml of rIFN-gamma for 48 hr increased the levels of H-2 expression and made both clones more resistant to NK-mediated lysis. In vivo studies with radiolabelled tumor cells showed that cells from the H-2+ clone survived better than cells from the H-2- clone in the pulmonary capillary bed after i.v. inoculation. This difference disappeared in mice ...Continue Reading

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Citations

Aug 26, 1998·International Journal of Cancer. Journal International Du Cancer·P L LolliniP Nanni
Jan 10, 2001·International Journal of Cancer. Journal International Du Cancer·A Garcia-LoraF Garrido
Jan 1, 1997·International Journal of Clinical & Laboratory Research·I AlgarraF Garrido
Jan 1, 1992·Immunologic Research·J Peña, R Solana
Mar 5, 2008·Cancer Immunology, Immunotherapy : CII·Karl-Johan MalmbergHans-Gustaf Ljunggren
Jul 14, 2010·Cancer Immunology, Immunotherapy : CII·Federico GarridoAngel M García-Lora
May 10, 2011·Cancer Immunology, Immunotherapy : CII·Cristina GarridoFederico Garrido
Apr 28, 2000·International Journal of Clinical & Laboratory Research·S PedrinaciF Garrido
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Jun 1, 2002·Human Immunology·Martine J JagerRolf Kiessling
Dec 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B L PetersenO Werdelin
Dec 5, 2014·International Journal of Cancer. Journal International Du Cancer·Federico GarridoAngel M Garcia-Lora
Aug 19, 1992·International Journal of Cancer. Journal International Du Cancer·M J MialdeaF Garrido
Jan 1, 1991·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·I AlgarraF Garrido
Dec 2, 1991·International Journal of Cancer. Journal International Du Cancer·J J GaforioF Garrido
May 28, 1993·International Journal of Cancer. Journal International Du Cancer·I AlgarraF Garrido
Feb 20, 1992·International Journal of Cancer. Journal International Du Cancer·H M BlottièreJ Le Pendu
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Angel Garcia-LoraFederico Garrido
Aug 1, 1989·Journal of Immunogenetics·A FerronF Garrido
Jul 6, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Stephen R GodingPer H Basse

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