NKX2-5 mutations in an inbred consanguineous population: genetic and phenotypic diversity

Scientific Reports
Ossama K Abou HassanGeorges M Nemer

Abstract

NKX2-5 mutations are associated with different forms of congenital heart disease. Despite the knowledge gained from molecular and animal studies, genotype-phenotype correlations in humans are limited by the lack of large cohorts and the incomplete assessment of family members. We hypothesized that studying the role of NKX2-5 in inbred populations with homogeneous genetic backgrounds and high consanguinity rates such as Lebanon could help closing this gap. We sequenced NKX2-5 in 188 index CHD cases (25 with ASD). Five variants (three segregated in families) were detected in eleven families including the previously documented p.R25C variant, which was found in seven patients from different families, and in one healthy individual. In 3/5 familial dominant ASD cases, we identified an NKX2-5 mutation. In addition to the heterogeneity of NKX2-5 mutations, a diversity of phenotypes occurred within the families with predominant ASD and AV block. We did in fact identify a large prevalence of Sudden Cardiac Death (SCD) in families with truncating mutations, and two patients with coronary sinus disease. NKX2-5 is thus responsible for dominant familial ASD even in consanguineous populations, and a wide genetic and phenotypic diversity is c...Continue Reading

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Citations

Jan 22, 2017·Genesis : the Journal of Genetics and Development·Jennifer M IkléDavid E Clouthier
Jan 28, 2020·Cardiology in Review·Yara A JelwanFrancine K Welty
May 6, 2020·Human Mutation·Jorge E KolomenskiLiliana Dain
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Dec 12, 2019·Journal of Cardiovascular and Thoracic Research·Samira KalayiniaNejat Mahdieh
Jul 28, 2020·Circulation Research·Antoinette F van OuwerkerkVincent M Christoffels
May 5, 2017·Scientific Reports·Athar KhalilGeorges Nemer

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Datasets Mentioned

BETA
SCV000188643

Methods Mentioned

BETA
Exome Sequencing

Software Mentioned

PROVEAN
Linkmap
Picard
Fastlink
SIFT
Genome Analysis Toolkit ( GATK )
PolyPhen

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