NleB/SseK effectors from Citrobacter rodentium, Escherichia coli, and Salmonella enterica display distinct differences in host substrate specificity.

The Journal of Biological Chemistry
Samir El QaidiPhilip R Hardwidge

Abstract

Many Gram-negative bacterial pathogens use a syringe-like apparatus called a type III secretion system to inject virulence factors into host cells. Some of these effectors are enzymes that modify host proteins to subvert their normal functions. NleB is a glycosyltransferase that modifies host proteins with N-acetyl-d-glucosamine to inhibit antibacterial and inflammatory host responses. NleB is conserved among the attaching/effacing pathogens enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli (EPEC), and Citrobacter rodentium Moreover, Salmonella enterica strains encode up to three NleB orthologs named SseK1, SseK2, and SseK3. However, there are conflicting reports regarding the activities and host protein targets among the NleB/SseK orthologs. Therefore, here we performed in vitro glycosylation assays and cell culture experiments to compare the activities and substrate specificities of these effectors. SseK1, SseK3, EHEC NleB1, EPEC NleB1, and Crodentium NleB blocked TNF-mediated NF-κB pathway activation, whereas SseK2 and NleB2 did not. C. rodentium NleB, EHEC NleB1, and SseK1 glycosylated host GAPDH. C. rodentium NleB, EHEC NleB1, EPEC NleB1, and SseK2 glycosylated the FADD (Fas-associated death domain protei...Continue Reading

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Citations

Jan 18, 2018·Infection and Immunity·Maarten F de Jong, Neal M Alto
Feb 17, 2018·The Journal of Biological Chemistry·Diego EspositoKatrin Rittinger
Sep 2, 2017·The Journal of Biological Chemistry·Nichollas E ScottElizabeth L Hartland
Mar 25, 2019·Molecular & Cellular Proteomics : MCP·Joshua P M NewsonElizabeth L Hartland
Mar 7, 2020·Microorganisms·Juan Luis Araujo-GarridoFrancisco Ramos-Morales
Jan 9, 2019·Frontiers in Cellular and Infection Microbiology·Samir El QaidiPhilip R Hardwidge
May 6, 2020·International Journal of Molecular Sciences·Juan Luis Araujo-GarridoFrancisco Ramos-Morales
May 16, 2020·Frontiers in Cellular and Infection Microbiology·Xing PanShan Li
Aug 9, 2020·Frontiers in Cell and Developmental Biology·Juan XueShan Li
Aug 14, 2019·Frontiers in Immunology·Madeleine A Wemyss, Jaclyn S Pearson
May 21, 2020·Frontiers in Cellular and Infection Microbiology·Juan XueShan Li
Jan 25, 2020·Scientific Reports·Samir El QaidiPhilip R Hardwidge
Sep 26, 2020·Frontiers in Cellular and Infection Microbiology·Jiyao GanCristina Giogha
Nov 9, 2018·Pathogens·Miaomiao WuPhilip R Hardwidge
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Feb 17, 2021·Scientific Reports·Samir El QaidiPhilip R Hardwidge
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Jun 1, 2019·Molecular & Cellular Proteomics : MCP·JoshuaP M NewsonElizabeth L Hartland
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May 8, 2021·Cellular Microbiology·Ipsita NandiBenjamin Aroeti

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