NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain

Biochemical and Biophysical Research Communications
M T CorasanitiA Finazzi-Agrò

Abstract

Treatment of neuroblastoma cultures with N-methyl-D-aspartate (NMDA) or human immunodeficiency virus type 1 (HIV-1) coat protein, gp120, induces significant cytotoxic effects which are reduced by leupeptin, E-64, N-Ac-Leu-Leu-norleucinal (ALLnL) as well as by N-Ac-Leu-Leu-normethioninal (ALLnM) and this suggests that activation of the Ca(2+)-dependent protease, calpain, is involved. The cell death induced by NMDA and gp120 appears to be of the necrotic type; in fact, analysis of DNA fragmentation by flow cytometry or agarose gel electrophoresis failed to demonstrate signs of apoptosis, such as the presence of apoptotic bodies or internucleosomal cleavage. Similar negative results were also obtained by studying the nuclear morphology of the cells with Hoechst 33258 staining. Altogether the data indicate that neuroblastoma cell death induced by NMDA and gp120 is of the necrotic type and this implicates calpain protease.

Citations

Feb 13, 2001·International Journal of Experimental Pathology·P W Vanderklish, B A Bahr
Jun 14, 2002·Biochimica Et Biophysica Acta·Tao LuRonald L Mellgren
Oct 2, 2002·Journal of Neurochemistry·Mauro MaccarroneAlessandro Finazzi-Agrò
Jul 24, 2001·Medical and Pediatric Oncology·M Annicchiarico-PetruzzelliG Melino
Jul 30, 2005·Journal of Biosciences·Hakan Ozdener
Feb 11, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jerel FieldsE Masliah
Jul 2, 2003·Journal of Neuropathology and Experimental Neurology·Zhen PangJames W Geddes
Jun 10, 1998·Biochemical and Biophysical Research Communications·M T CorasanitiA Finazzi-Agrò

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