PMID: 8953570Dec 1, 1996Paper

NMDA excitotoxicity and free radical generation in rat brain homogenates: application of a chemiluminescence assay

Neurochemical Research
B KüçükkayaA S Yalçin

Abstract

NMDA, the specific agonist of glutamate gated ion channels permeable to calcium, is implicated as a causal factor in the pathogenesis of several neurobiological disorders such as stroke, seizures, ischemia, and chronic neurodegenerative diseases. On the other hand, evidence on the roles of oxidative mechanisms involved in NMDA-induced neurotoxicity is accumulating. In this study, we have used chemiluminescence measurements as an easy, rapid and sensitive assay to investigate the effects of NMDA and oxidative stress on brain cell vulnerability. Rat brain homogenates were incubated with increasing concentrations of glutamate and NMDA. Production of reactive oxygen species was followed by single photon emission measurements using the specific enhancers luminol and lucigenin. Increases in emission were observed at excitotoxic concentrations of glutamate and NMDA. Other parameters of oxidative stress such as diene conjugates, TBARS and carbonyl groups were also investigated. Our results indicated that chemiluminescence measurements may be used to study involvement of oxidative stress in neurotoxicity.

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Citations

Jul 2, 2003·Neurotoxicity Research·Lori K KlaidmanFred Samson
Apr 26, 2005·Journal of Neuroscience Methods·Yngo J GarciaNancy Peñaloza
Nov 24, 1999·Drugs in R&D
Feb 19, 2002·Brain Research Bulletin·María Dolores MayasJosé Manuel Martínez-Martos
Feb 4, 1999·Journal of Neurochemistry·J Y KohJ H Seo
Jun 19, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Keiko Funa, Masakiyo Sasahara
Mar 13, 2003·Redox Report : Communications in Free Radical Research·Chung-Shi YangJon-Son Kuo
Jul 1, 2004·Critical Reviews in Biotechnology·Zhonghu BaiBrian McNeil
Jun 30, 2006·Journal of Neurochemistry·Yoko IshiiMasakiyo Sasahara
May 3, 2005·Neurochemistry International·Svatava KasparováDusan Dobrota

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