Abstract
Both excitotoxicity and oxidative stress are implicated in the pathophysiology of central nervous system (CNS) ischaemia-reperfusion injury whereby astrocytes offer neural protection through the production of endogenous antioxidants and removal of glutamate from the extracellular milieu. This study investigated whether exogenous alpha-tocopherol, an antioxidant, could prevent N-methyl-D-aspartate (NMDA)-produced increases of the glial specific proteins, glutamine synthetase (GS) and glial fibrillary acidic protein (GFAP) in rat brain spheroids in vitro. NMDA (320 microM; 3 days in vitro (DIV)) was unable to induce lipid peroxidation in rat brain spheroids implying that excitotoxicity in this system did not involve substantial free radical formation. However at non-cytotoxic concentrations, increases in astroglial GS were prevented by alpha-tocopherol treatment, suggesting a role for ROS in the excitotoxic process. In contrast, NMDA-induced increases in GFAP remained unchanged by alpha-tocopherol indicating that oxidative stress may not be involved in reactive gliosis at non-cytotoxic NMDA concentrations.
References
Mar 25, 1977·Science·A Martinez-HernandezM D Norenberg
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C N OliverR A Floyd
Feb 1, 1991·Toxicology and Applied Pharmacology·M A PhilbertH E Lowndes
Jun 1, 1985·Journal of Neuroimmunology·L F Eng
Feb 1, 1986·Annals of Neurology·S M Rothman, J W Olney
Jul 19, 1988·Brain Research·N F Schor
Oct 1, 1987·Journal of Neurochemistry·J A DykensE Trenkner
Nov 1, 1993·Trends in Neurosciences·C W Olanow
Aug 1, 1995·Journal of the Neurological Sciences·Y PanH Jiang
Aug 1, 1994·Journal of Neurochemistry·J A Dykens
May 7, 1993·Brain Research·S C Bondy, D K Lee
Apr 1, 1993·Journal of Neurochemistry·A Frandsen, A Schousboe
Aug 1, 1996·Trends in Neurosciences·C Steinhäuser, V Gallo
Jun 7, 2005·Expert Opinion on Drug Safety·James P O'Callaghan, Krishnan Sriram
Sep 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C K AtterwillS Williams
Citations
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R P Wilson, P L Fowlkes
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D WigginsH A Krebs
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·I V Deaciuc, M Papadakis
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F J García-PalmerA Palou
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T MatsunoH Suzuki
May 22, 2002·Neurochemistry International·I SuárezB Fernández
Jun 16, 2000·Neuroscience Letters·G M HayesG E Griffin
Mar 22, 2002·The International Journal of Biochemistry & Cell Biology·José M MatésJavier Márquez
Jun 20, 2008·Neurotoxicology·André-Guilhem CalasCéline Montécot
Sep 7, 2000·Glia·B VivianiM Marinovich
Mar 1, 2000·Glia·H TumaniR Nau
Jan 20, 2017·Frontiers in Pharmacology·James YipYuchuan Ding