NMDA receptor-mediated currents in rat cerebellar granule and unipolar brush cells

Journal of Neurophysiology
Daniela BillupsN T Slater

Abstract

The properties of N-methyl-D-aspartate (NMDA) receptor-mediated currents at the giant cerebellar mossy-fiber unipolar brush cell (UBC) synapse were compared with those of adjacent granule cells using patch-clamp recording methods in thin slices of rat cerebellar nodulus. In UBCs, NMDA receptor-mediated excitatory postsynaptic currents (EPSCs) decayed as a single exponential whose time constant was independent of membrane potential. The EPSC was reduced in all cells by the NR1/NR2B-selective antagonist ifenprodil, and the Zn(2+) chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) produced a transient potentiation in 50% of cells. In contrast, the NMDA EPSC in granule cells decayed as a double exponential that dramatically switched to a slower rate at positive membrane potentials. The synaptic response in some granule cells also displayed a late second peak at positive potentials, and in others, activation of mossy fibers produced repetitive trains of EPSCs indicating they may be postsynaptic to the UBC network. Single-channel recordings of outside-out somatic patches from UBCs in magnesium-free solution revealed only high-conductance (50 pS) channels whose open time was increased with depolarization, but the openi...Continue Reading

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Citations

Oct 22, 2008·The Journal of Physiology·Richard J Clarke, Jon W Johnson
Mar 24, 2007·The Journal of Physiology·Marco J RussoMarco Martina
Jun 9, 2006·Progress in Neurobiology·Masao Ito
Aug 17, 2012·Nature Reviews. Neuroscience·Zhenyu GaoChris I De Zeeuw
Mar 20, 2015·American Journal of Physiology. Cell Physiology·Pascal HänggiAnna Bogdanova
May 7, 2020·Frontiers in Computational Neuroscience·Lingling AnJian K Liu
Oct 13, 2010·Brain Research Reviews·Enrico MugnainiMarco Martina

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