NMDA-receptor mediated efflux of N-acetylaspartate: physiological and/or pathological importance?

Neurochemistry International
Mattias TranbergMats Sandberg

Abstract

N-Acetylaspartate (NAA) is a largely neuron specific dianionic amino acid present in high concentration in vertebrate brain. Many fundamental questions concerning N-acetylaspartate in brain remain unanswered. One such issue is the predominantly neuronal synthesis and largely glial catabolism which implies the existence of a regulated efflux from neurons. Here we show that transient (5 min) NMDA-receptor activation (60 microM) induces a long lasting Ca2+ -dependent efflux of N-acetylaspartate from organotypic slices of rat hippocampus. The NMDA-receptor stimulated efflux was unaffected by hyper-osmotic conditions (120 mM sucrose) and no efflux of N-acetylaspartate was evoked by high K+ -depolarization (50 mM) or kainate (300 microM). These results indicate that the efflux induced by NMDA is not related directly to either cell swelling or depolarization but is coupled to Ca2+ -influx via the NMDA-receptor. The efflux of N-acetylaspartate persisted at least 20 min after the omission of NMDA, similar to the efflux of the organic anions glutathione and phosphoethanolamine. The efflux of taurine and hypotaurine was also stimulated by NMDA but returned more quickly to basal levels. The NMDA-receptor stimulated efflux of N-acetylaspart...Continue Reading

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Citations

Aug 7, 2008·Journal of Neuroscience Research·José A Rodríguez-NavarroJosé M Solís
Jul 1, 2014·Pediatrics International : Official Journal of the Japan Pediatric Society·Hideki Hoshino, Masaya Kubota
May 2, 2006·Molecular and Cellular Endocrinology·Aryan M A NamboodiriChikkathur N Madhavarao
Jul 16, 2005·Analytical Biochemistry·Mattias TranbergMats Sandberg
Oct 19, 2016·Journal of Neuroscience Research·Mary C McKennaArne Schousboe
Apr 3, 2007·Neurochemical Research·Mattias TranbergMats Sandberg
Sep 29, 2018·Molecular Psychiatry·Seico BennerHidenori Yamasue
Sep 29, 2020·Frontiers in Neurology·Jingying WuLi Cao

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