NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions

Plant Biotechnology Journal
Roxana PortielesOrlando Borrás-Hidalgo

Abstract

Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range of microbes. In this article, we describe NmDef02, a novel cDNA encoding a putative defensin isolated from Nicotiana megalosiphon upon inoculation with the tobacco blue mould pathogen Peronospora hyoscyami f.sp. tabacina. NmDef02 was heterologously expressed in the yeast Pichia pastoris, and the purified recombinant protein was found to display antimicrobial activity in vitro against important plant pathogens. Constitutive expression of NmDef02 gene in transgenic tobacco and potato plants enhanced resistance against various plant microbial pathogens, including the oomycete Phytophthora infestans, causal agent of the economically important potato late blight disease, under greenhouse and field conditions.

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Citations

Aug 21, 2013·PloS One·Benjamin ZeitlerChristian Lindermayr
Oct 5, 2013·Folia Microbiologica·Robert NawrotAnna Goździcka-Józefiak
May 9, 2014·Applied Microbiology and Biotechnology·Hui-Yu YiXiao-Qiang Yu
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Sep 30, 2021·Biotechnology and Applied Biochemistry·Mine Erdem Büyükkiraz, Zülal Kesmen

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