PMID: 9531426Apr 8, 1998Paper

NMR analysis of a potent decapeptide agonist of human C5a anaphylatoxin

The Journal of Peptide Research : Official Journal of the American Peptide Society
S M VogenS A Sherman

Abstract

A NMR investigation in H2O, TFE and DMSO of a conformationally constrained, potent decapeptide agonist of human C5a, YSFKDMPLaR (C5a65-74, Y65, F67, P71, D-Ala73) showed that its N-terminal region (YSFKD) exhibited an extended backbone conformation in H2O and a more twisted conformation in both TFE/H2O (30:70, v/v; referred to as TFE) and DMSO. The C-terminal region (MPLaR) of the peptide adopted compact, turn-like structures. In H2O, the C-terminal region adopted a type II beta-turn or a distorted type V/II beta-turn involving residues PLaR. In the distorted type V/II beta-turn, Leu72 exhibited a conformation typical of a type V beta-turn, whereas D-Ala73 exhibited a conformation typical of a type II beta-turn. The distorted type V/II beta-turn overlapped with an inverse gamma-turn involving residues MPL. In DMSO, the C-terminal region had the analogous inverse gamma-turn and the V/II beta-turn found in H2O. In many of the DMSO structures, two inverse gamma-turns in the MPL and PLa positions formed a double-inverse gamma-turn. None of the turns observed in H2O were present in TFE. However, in TFE, the PLa residues formed an inverse gamma-turn. Overall, the turn-like structural motifs in the C-terminal region of the peptide in ...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Jan 1, 1980·CRC Critical Reviews in Biochemistry·J A Smith, L G Pease
Sep 1, 1995·Journal of Medicinal Chemistry·S D SandersonS M Taylor

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Citations

Apr 9, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·S M VogenS D Sanderson
Dec 21, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·M DhanasekaranO Prakash
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·S M VogenS A Sherman

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