NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

Molecular BioSystems
C AiroldiP Coccetti


Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.


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