NMR analysis of neutrophil activation in sputum samples from patients with cystic fibrosis

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Erik J SaudeRedwan Moqbel

Abstract

Disorders of the respiratory system, such as cystic fibrosis (CF), involve the infiltration and activation of airway inflammatory cells, including neutrophils. This leads to the secretion of peroxidases, which react further with substrates in solution to produce oxidative metabolites, such as 3-chlorotyrosine. Elevated levels of modified tyrosine residues in the airways of patients with CF may be detectable by nuclear magnetic resonance (NMR) in correlation with inflammatory cell influx. In this study, high-resolution (500 MHz) 1H NMR was used to analyze the production of modified tyrosine residues resulting from in vitro stimulation of peripheral blood eosinophils and neutrophils, as well as in sputum samples from control subjects and patients with CF. Following in vitro stimulation, purified peripheral blood neutrophils generated 3-chlorotyrosine, while eosinophils produced predominantly 3-bromotyrosine and 3,5-dibromotyrosine. Chlorinated and brominated tyrosine residues were detected in sputum samples from patients with CF (N=7), but were not detected in the control group (N=9). Neutrophil counts in CF sputum correlated strongly with the presence of 3-chlorotyrosine (r2=0.869). Our findings indicate that neutrophil and eosi...Continue Reading

References

Dec 1, 1990·Thorax·J S Elborn, D J Shale
Sep 1, 1980·Annals of Internal Medicine·S J Klebanoff
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·M M PizzichiniF E Hargreave
Nov 1, 1996·American Journal of Respiratory and Critical Care Medicine·G M GauvreauP M O'Byrne
May 1, 1997·American Journal of Respiratory and Critical Care Medicine·M M PizzichiniF E Hargreave
Aug 1, 1997·Current Opinion in Cell Biology·S Devidas, W B Guggino
Feb 11, 1998·Lancet·B J Rosenstein, P L Zeitlin
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·E PizzichiniF E Hargreave
May 17, 2000·The Journal of Clinical Investigation·W WuS L Hazen
Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·A Van Der VlietC E Cross
Apr 20, 2002·Current Opinion in Allergy and Clinical Immunology·P Lacy, R Moqbel
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paige LacyRedwan Moqbel
Oct 1, 2003·The European Respiratory Journal·N ReinhardtK P Paul
Dec 9, 2003·Amino Acids·C GiuliviK J A Davies

❮ Previous
Next ❯

Citations

Nov 8, 2011·Chemical Research in Toxicology·Tessa J Barrett, Clare L Hawkins
Mar 12, 2008·Antioxidants & Redox Signaling·Michael J DaviesMartin D Rees
Oct 22, 2008·American Journal of Respiratory and Critical Care Medicine·Erik J SaudeDarryl J Adamko
Oct 11, 2012·Bioanalysis·Stuart SnowdenCraig E Wheelock
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·O M PanasenkoA V Sokolov
Oct 7, 2011·Intensive Care Medicine·José L Izquierdo-GarcíaJosé A Lorente
May 15, 2012·Free Radical Biology & Medicine·Jun YangBruce D Hammock
Apr 15, 2008·Biochemical and Biophysical Research Communications·Matthew Whiteman, Jeremy P E Spencer
Nov 24, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D S Wishart
Dec 20, 2005·The Journal of Surgical Research·Imran MohiuddinChangyi Chen
Nov 19, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·B Fatemeh Nobakht M GhAfsaneh Arefi Oskouie
Mar 8, 2011·The Journal of Allergy and Clinical Immunology·Erik J SaudeDarryl J Adamko
Mar 30, 2007·Journal of Separation Science·Rania BakryGünther K Bonn
Nov 30, 2005·Electrophoresis·Christian W HuckGünther K Bonn
Mar 14, 2014·Molecular BioSystems·Lyn H JonesErik C Hett
Sep 19, 2019·Free Radical Research·Mariam SabirAli R Mani
Aug 30, 2005·Archives of Biochemistry and Biophysics·Revathy Senthilmohan, Anthony J Kettle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.