NMR and crystallographic structural studies of the Elongation factor P from Staphylococcus aureus.

European Biophysics Journal : EBJ
A A GolubevK S Usachev

Abstract

Elongation factor P (EF-P) is a translation protein factor that plays an important role in specialized translation of consecutive proline amino acid motifs. EF-P is an essential protein for cell fitness in native environmental conditions. It regulates synthesis of proteins involved in bacterial motility, environmental adaptation and bacterial virulence, thus making EF-P a potential drug target. In the present study, we determined the solution and crystal structure of EF-P from the pathogenic bacteria Staphylococcus aureus at 1.48 Å resolution. The structure can serve as a platform for structure-based drug design of novel antibiotics to combat the growing antibiotic resistance of S. aureus.

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Citations

May 22, 2020·FEBS Open Bio·Alexander GolubevKonstantin Usachev

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Methods Mentioned

BETA
NMR
X-ray
gel filtration
nuclear magnetic resonance
size-exclusion chromatography

Software Mentioned

PROCHECK
COOT
Phaser
ARIA2
TALOS
CNS ( Crystallography and NMR System
Chimera
UCSF Chimera
Phenix
XDS

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