NMR and EPR structural delineation of copper(II) complexes formed by kanamycin A in water

Dalton Transactions : an International Journal of Inorganic Chemistry
Nicola D'AmelioWojciech Szczepanik

Abstract

The complexes formed by kanamycin A at three different pH values (5.5, 7.4 and 12.0) were investigated by NMR and EPR spectroscopy. Paramagnetic relaxation contributions to proton relaxation rates were measured using a combination of the TOCSY sequence with the inversion recovery experiment in order to gain signal resolution in the bulk region. Measured contributions were converted into distances and used for structural determination by restrained simulated annealing where all possible chair and boat conformations of the rings were taken into account. The interaction of the Cu(II) ion with the nitrogen of the C ring is apparent at all pH values. At higher pH also the amino group of ring A starts to be involved in the metal coordination sphere. This is accompanied by a switch in conformation of ring C. Structures are consistent with the involvement in the coordination sphere either of the 2' or 4' hydroxyl oxygens at pH 5.5 and the 5 and the 6' hydroxyl oxygens (or the ring oxygen) at pH 12.0.

References

Mar 1, 1967·Japanese Journal of Pharmacology·S Yamabe
Nov 1, 1983·Antimicrobial Agents and Chemotherapy·S F QueenerF G Hamel
Feb 1, 1982·The Journal of Antimicrobial Chemotherapy·K Shannon, I Phillips
Nov 21, 1997·Journal of Molecular Biology·P GüntertK Wüthrich
Mar 23, 1999·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·E GaggelliM Delfini
Apr 21, 1999·Carbohydrate Research·M Jezowska-BojczukH Kozłowski
Apr 11, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·A Sreedhara, J A Cowan
May 25, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·A Sreedhara, J A Cowan
May 26, 2001·Journal of Inorganic Biochemistry·M Jezowska-BojczukW Bal
Jul 18, 2002·Chemical Communications : Chem Comm·Chun-An Chen, J A Cowan
Apr 2, 2003·Journal of Inorganic Biochemistry·Wojciech SzczepanikMalgorzata Jezowska-Bojczuk
Sep 30, 1958·Annals of the New York Academy of Sciences·H UMEZAWA

❮ Previous
Next ❯

Citations

Dec 7, 2007·Chembiochem : a European Journal of Chemical Biology·Duccio BalenciGianni Valensin
Sep 15, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Elena GaggelliMałgorzata Jeżowska-Bojczuk
Feb 14, 2006·Dalton Transactions : an International Journal of Inorganic Chemistry·Magdalena SwiatekMałgorzata Jezowska-Bojczuk
Mar 27, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·D BalenciM Jezowska-Bojczuk
Mar 3, 2011·Journal of Molecular Modeling·Johannes M DieterichBernd Hartke
Jan 11, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Anjali Patwardhan, J A Cowan
Oct 2, 2013·The Journal of Physical Chemistry. B·Nicola D'AmelioIvan Donati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.