NMR and MS-based Stable Isotope-Resolved Metabolomics and Applications in Cancer Metabolism

Trends in Analytical Chemistry : TRAC
Andrew N LaneTeresa W-M Fan

Abstract

There is considerable interest in defining metabolic reprogramming in human diseases, which is recognized as a hallmark of human cancer. Although radiotracers have a long history in specific metabolic studies, stable isotope-enriched precursors coupled with modern high resolution mass spectrometry and NMR spectroscopy have enabled systematic mapping of metabolic networks and fluxes in cells, tissues and living organisms including humans. These analytical platforms are high in information content, are complementary and cross-validating in terms of compound identification, quantification, and isotope labeling pattern analysis of a large number of metabolites simultaneously. Furthermore, new developments in chemoselective derivatization and in vivo spectroscopy enable tracking of labile/low abundance metabolites and metabolic kinetics in real-time. Here we review developments in Stable Isotope Resolved Metabolomics (SIRM) and recent applications in cancer metabolism using a wide variety of stable isotope tracers that probe both broad and specific aspects of cancer metabolism required for proliferation and survival.

Citations

Jul 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Ho SeoHong-Seok Son
Sep 24, 2020·International Journal of Molecular Sciences·Aneta BalcerczykGilles J P Rautureau
Dec 16, 2020·Proteomics·Timothy D Veenstra
Mar 28, 2020·Genes & Diseases·Andrew N LaneTeresa W-M Fan
Apr 8, 2021·Annual Review of Analytical Chemistry·Cassio LimaRoyston Goodacre
Nov 7, 2020·Analytical Chemistry·Marine P M LetertrePatrick Giraudeau

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