NMR approach to the quantification of nonstatistical 13C distribution in natural products: vanillin

Analytical Chemistry
Eve TenailleauSerge Akoka

Abstract

Quantitative (13)C NMR conditions have been established that permit the precise determination of site-specific (13)C/(12)C ratios at low or natural abundance. Spectral acquisition parameters have been optimized in order to obtain minimum intensity distortions over the spectral width and in relation to the major sources of inaccuracy: the relaxation times, the decoupling pulse and power, and nuclear Overhauser effects. A major reduction in experimental time resulting from a study of the relaxation times and variance analysis has been achieved. The influence of (1)H decoupling conditions on peak areas was shown to be critical in that different relative peak areas are obtained according to the decoupling power. The efficiency with which the quantitative (13)C NMR method can determine site-specific (13)C/(12)C ratios in natural products has been tested for 12 independent samples of vanillin from different sources. Discriminatory analysis performed in the space defined by the site-specific carbon isotope ratios allows natural vanillin and that from different synthetic origins to be unambiguously distinguished.

References

Jul 15, 1977·Science·R R ArnoldJ R McGhee
Oct 17, 2002·Journal of Agricultural and Food Chemistry·Fabienne F BensaidGérard J Martin

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Citations

Jan 5, 2016·Journal of Asian Natural Products Research·Jun-Jun GeXiao-Xia Ye
Dec 25, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Gérald S RemaudSerge Akoka
Dec 5, 2006·Journal of Magnetic Resonance·Eve Tenailleau, Serge Akoka
Jan 10, 2017·Nature Communications·Maria Theresia PöschkoHervé Desvaux
Sep 22, 2007·International Journal of Food Sciences and Nutrition·Arun K SinhaNandini Sharma
Dec 7, 2016·Magnetic Resonance in Chemistry : MRC·Tangi JézéquelSerge Akoka
Jul 1, 2020·Amino Acids·Cornelia Rasmussen, David W Hoffman
Sep 13, 2015·Journal of the Science of Food and Agriculture·Yulia B Monakhova, Bernd Wk Diehl
Jan 28, 2021·Analytical and Bioanalytical Chemistry·Fernando G M ViolanteFrancisco Radler de Aquino Neto
Jun 19, 2018·Analytical Chemistry·Valentin JoubertGérald S Remaud
Apr 16, 2013·Analytical Chemistry·Estelle MartineauPatrick Giraudeau
Nov 8, 2019·Analytical Chemistry·David W Hoffman, Cornelia Rasmussen
Dec 23, 2004·Journal of Agricultural and Food Chemistry·Eve J TenailleauSerge Akoka
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Francois GuyonBernard Medina

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