NMR artifacts caused by decoupling of multiple-spin coherences: improved SLAP experiment

Magnetic Resonance in Chemistry : MRC
Vratislav Blechta, Jan Schraml

Abstract

Contrary to common expectations, multiple-spin coherences containing products of proton and heteronucleus operators (e.g. Hu Cx , u = x, y, z) can produce not only sidebands but also noticeable centerband NMR signals of the heteronucleus during acquisition under 1H broadband decoupling. Such centerband signals of low abundant heteronuclei can be sources of relatively strong unexpected artifacts in NMR experiments that aim to detect very weak signals from much less-abundant isotopomers, e.g. 13C-13C ones. These findings lead to a new design of Sign Labeled Polarization Transfer (SLAP) pulse sequence (MSS-SLAP) with improved suppression of centerband peaks that are because of singly, e.g. 13C, labeled molecules (parent peaks). The MSS-SLAP experiment and its MSS-BIRD-SLAP variant are compared with a few older SLAP versions.

References

Jan 4, 1998·Journal of Magnetic Resonance·M R Bendall, T E Skinner
Apr 18, 2013·Magnetic Resonance in Chemistry : MRC·Vratislav Blechta, Jan Schraml
Sep 13, 2013·Magnetic Resonance in Chemistry : MRC·Vratislav Blechta, Jan Schraml

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