NMR-based metabolic profiling of urine, serum, fecal, and pancreatic tissue samples from the Ptf1a-Cre; LSL-KrasG12D transgenic mouse model of pancreatic cancer

PloS One
Michelle J SchmahlMichael A Kennedy

Abstract

Pancreatic cancer is the third leading cause of cancer deaths in the United States with more than 53,000 expected to be diagnosed with the disease in 2018. The median survival time after diagnosis is four to six months. The poor survival statistics are due in part to the fact that pancreatic cancer is typically asymptomatic until it reaches advanced stages of the disease. Although surgical resection provides the best chance of survival, pancreatic cancer is rarely detected when surgery is still possible due, in part, to lack of effective biomarkers for early detection. The goal of the research reported here was to determine if it was possible to identify metabolic biomarkers for detection of pre-cancerous pancreatic intraepithelial neoplasia (PanIN) that precede pancreatic adenocarcinoma. The transgenic Ptf1a-Cre; LSL-KrasG12D mouse strain was used as a model of pancreatic cancer progression. Nuclear magnetic resonance (NMR) spectroscopy was employed to compare metabolic profiles of urine, sera, fecal extracts, and pancreatic tissue extracts collected from control and study mice aged 5, 11, and 15 months, including 47 mice with tumors. We were able to identify the following potential biomarkers: decreased 3-indoxylsulfate, benz...Continue Reading

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Citations

Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·William C Joesten, Michael A Kennedy
Nov 7, 2019·Metabolites·Irina PetrovaMichael A Kennedy
Oct 20, 2020·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Ilana R Azulay ChertokMichael Kennedy
Apr 13, 2021·American Journal of Physiology. Renal Physiology·Stephen W StandageMichael A Kennedy

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Methods Mentioned

BETA
NMR
transgenic
dissections
genotyping
PCR
electrophoresis
PCA
Profiler
deamination

Software Mentioned

PLS
Metabolome
excel
DA
SIMCA
R Project for Statistical Computing ( R )
AMIX
R
Topspin
ChenomX Profiler

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