NMR-based metabolomic techniques identify potential urinary biomarkers for early colorectal cancer detection

Oncotarget
Zhening WangJurong Yang

Abstract

Better early detection methods are needed to improve the outcomes of patients with colorectal cancer (CRC). Proton nuclear magnetic resonance spectroscopy (1H-NMR), a potential non-invasive early tumor detection method, was used to profile urine metabolites from 55 CRC patients and 40 healthy controls (HCs). Pattern recognition through orthogonal partial least squares-discriminant analysis (OPLS-DA) was applied to 1H-NMR processed data. Model specificity was confirmed by comparison with esophageal cancers (EC, n=18). Unique metabolomic profiles distinguished all CRC stages from HC urine samples. A total of 16 potential biomarker metabolites were identified in stage I/II CRC, indicating amino acid metabolism, glycolysis, tricarboxylic acid (TCA) cycle, urea cycle, choline metabolism, and gut microflora metabolism pathway disruptions. Metabolite profiles from early stage CRC and EC patients were also clearly distinguishable, suggesting that upper and lower gastrointestinal cancers have different metabolomic profiles. Our study assessed important metabolomic variations in CRC patient urine samples, provided information complementary to that collected from other biofluid-based metabolomics analyses, and elucidated potential underly...Continue Reading

Citations

Dec 4, 2019·Expert Review of Gastroenterology & Hepatology·Jiaying YuLiyan Liu
May 17, 2019·Nature Reviews. Urology·Sarah S DingesLeo L Cheng
Jun 16, 2019·Internal and Emergency Medicine·Nooshin DaliliShiva Kalantari
Jun 12, 2020·World Journal of Gastroenterology : WJG·Giulia NanniniLeonardo Tenori
Feb 28, 2020·World Journal of Gastrointestinal Oncology·Alexandre Loktionov
Aug 3, 2018·Journal of Assisted Reproduction and Genetics·Maria Antonietta Castiglione MorelliAngela Ostuni
Mar 4, 2021·Analytical Chemistry·Rajat DhyaniNaveen Kumar Navani
Mar 25, 2021·Critical Reviews in Analytical Chemistry·Younes AftabiAbolghasem Jouyban
Jul 30, 2021·World Journal of Gastrointestinal Oncology·Hande BeklenKazim Yalcin Arga
Nov 23, 2021·Analytical Chemistry·Ana M SalmerónIgnacio Fernández

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Methods Mentioned

BETA
PCA
NMR

Software Mentioned

SIMCA
P +
SPSS
OPLS
DA

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