PMID: 9430608Jan 31, 1998Paper

NMR detection of 13CH313COOH from 3-13C-glucose: a signature for Bifidobacterium fermentation in the intestinal tract

The Journal of Nutrition
M J WolinT L Miller

Abstract

The gastrointestinal tracts of breast-fed infants are colonized more easily with bifidobacteria than are those of formula-fed infants. Colonization is thought to reduce infant diarrhea. Amendments to formulas that improve colonization by bifidobacteria are being actively investigated. Colonization studies almost invariably require measurements of the concentration of the bifidobacteria in feces to assess their importance in the colon. We investigated the use of nuclear magnetic resonance (NMR) analysis of products of fermentations of 1- and 3-13C-glucose to evaluate the importance of bifidobacteria in the colonic ecosystem. Bifidobacteria use a unique pathway of hexose catabolism to produce primarily acetate and lactate. The fermentation yields 3 mol of acetate from 2 mol of glucose. Two of the acetates are formed from C1 and C2 of glucose and the third is formed entirely from C3 of glucose. We first employed high resolution NMR to verify the pathway used by a pure culture of Bifidobacterium bifidum. The major products of fermentation of 1- and 3-13C-glucose were acetate and lactate. Most of the 13C from 3-13C-glucose was in 13CH313COOH with equal enrichment in the methyl and carboxyl groups. The 13C-acetate from 1-13C-glucose ...Continue Reading

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Citations

Oct 1, 2013·Applied and Environmental Microbiology·Irene González-RodríguezAna Rute Neves
Jul 25, 2019·Applied Microbiology and Biotechnology·Ravindra Pal Singh
Jul 14, 2009·NMR in Biomedicine·Albert A de GraafKoen Venema
Dec 9, 2014·Bioscience, Biotechnology, and Biochemistry·Takuya AkiyamaHiroshi Hatano
Feb 7, 2001·The American Journal of Clinical Nutrition·A Bezkorovainy
May 17, 2003·The Proceedings of the Nutrition Society·David L ToppingAnthony R Bird
Jan 14, 2021·Biotechnology and Bioengineering·Robert D HoelzleDamien Batstone
Feb 23, 2021·Frontiers in Microbiology·Sandra M KellyDouwe van Sinderen
Nov 10, 2010·Journal of Proteome Research·Yumiko NakanishiJun Kikuchi

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