NMR-Guided Mass Spectrometry for Absolute Quantitation of Human Blood Metabolites

Analytical Chemistry
G A Nagana GowdaDaniel Raftery

Abstract

Broad-based, targeted metabolite profiling using mass spectrometry (MS) has become a major platform used in the field of metabolomics for a variety of applications. However, quantitative MS analysis is challenging owing to numerous factors including (1) the need for, ideally, isotope-labeled internal standards for each metabolite, (2) the fact that such standards may be unavailable or prohibitively costly, (3) the need to maintain the standards' concentrations close to those of the target metabolites, and (4) the alternative use of time-consuming calibration curves for each target metabolite. Here, we introduce a new method in which metabolites from a single serum specimen are quantified on the basis of a recently developed NMR method [ Nagana Gowda et al. Anal. Chem. 2015 , 87 , 706 ] and then used as references for absolute metabolite quantitation using MS. The MS concentrations of 30 metabolites thus derived for test serum samples exhibited excellent correlations with the NMR ones (R2 > 0.99) with a median CV of 3.2%. This NMR-guided-MS quantitation approach is simple and easy to implement and offers new avenues for the routine quantification of blood metabolites using MS. The demonstration that NMR and MS data can be compar...Continue Reading

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Citations

Jun 23, 2020·Annual Review of Biochemistry·Emma J FenechMaya Schuldiner
Aug 12, 2020·Aging Cell·Jeremy A WhitsonPeter S Rabinovitch
May 3, 2020·Metabolomics : Official Journal of the Metabolomic Society·Nikolaos G BliziotisLeo A J Kluijtmans
Jul 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongying DuJie Wang
Oct 6, 2020·The Journal of Trauma and Acute Care Surgery·Dara L HornGrant E O'Keefe
Nov 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra A Crook, Robert Powers
Mar 21, 2021·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Alireza Salehi MMikael Landén
Nov 7, 2020·Analytical Chemistry·Marine P M LetertrePatrick Giraudeau
Nov 13, 2020·Analytical Chemistry·Arthur S EdisonSicong Zhang
Jul 13, 2019·Journal of Agricultural and Food Chemistry·Zhiyuan MengWentao Zhu

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