NMR insights into folding and self-association of Plasmodium falciparum P2.

PloS One
Pushpa MishraRamakrishna V Hosur

Abstract

The eukaryotic 60S-ribosomal stalk is composed of acidic ribosomal proteins (P1 and P2) and neutral protein P0, which are thought to be associated as a pentameric structure, [2P1, 2P2, P0]. Plasmodium falciparum P2 (PfP2) appears to play additional non-ribosomal functions associated with its tendency for homo-oligomerization. Recombinant bacterially expressed PfP2 protein also undergoes self-association, as shown by SDS-PAGE analysis and light scattering studies. Secondary structure prediction algorithms predict the native PfP2 protein to be largely helical and this is corroborated by circular dichroism investigation. The (1)H-(15)N HSQC spectrum of native P2 showed only 43 cross peaks compared to the expected 138. The observed peaks were found to belong to the C-terminal region, suggesting that this segment is flexible and solvent exposed. In 9 M urea denaturing conditions the chain exhibited mostly non-native β structural propensity. (15)N Relaxation data for the denatured state indicated substantial variation in ms-µs time scale motion along the chain. Average area buried upon folding (AABUF) calculations on the monomer enabled identification of hydrophobic patches along the sequence. Interestingly, the segments of slower mo...Continue Reading

References

Oct 1, 1978·European Journal of Biochemistry·A T GudkovV N Bushuev
Feb 1, 1975·Biophysical Chemistry·O B Ptitsyn, A A Rashin
Feb 1, 1992·Trends in Genetics : TIG·F A Van Engelen, S C De Vries
Jan 1, 1990·Annual Review of Biochemistry·P S Kim, R L Baldwin
Aug 27, 1990·Biochimica Et Biophysica Acta·M T Saenz-RoblesJ P Ballesta
Jul 1, 1989·Trends in Biochemical Sciences·R L Baldwin
Jun 5, 1987·Journal of Molecular Biology·M Leijonmarck, A Liljas
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T UchiumiR R Traut
Aug 30, 1985·Science·G D RoseM H Zehfus
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R Fersht
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E OtzenA R Fersht
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Shortle
Dec 1, 1995·Computer Applications in the Biosciences : CABIOS·C Geourjon, G Deléage
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S M FreundA R Fersht
Nov 1, 1996·Protein Science : a Publication of the Protein Society·J O Wrabl, D Shortle
Apr 29, 1997·Biochemistry·T KiefhaberC Wagner
Nov 5, 1997·Current Opinion in Structural Biology·A G Palmer
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G DonneH J Dyson
Feb 12, 1998·Journal of Molecular Biology·C J PenkettL J Smith
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R K AgrawalJ Frank
Jul 17, 1998·Nature Structural Biology·L E Kay
Mar 20, 1999·Biochimica Et Biophysica Acta·J W TannerP A Liebman
Mar 24, 1999·Trends in Biochemical Sciences·R L Baldwin, G D Rose
Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·M TchórzewskiN Grankowski
Jul 13, 2000·Protein Science : a Publication of the Protein Society·M Ouali, R D King
Aug 31, 2000·Nature Structural Biology·R Ishima, D A Torchia
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Mar 2, 2002·Science·Judith Klein-SeetharamanHarald Schwalbe
Jun 11, 2002·Biochemical and Biophysical Research Communications·Amarnath ChatterjeeRamakrishna V Hosur
Oct 16, 2002·Biochemistry·Stephan SchwarzingerH Jane Dyson
Nov 7, 2002·Advances in Protein Chemistry·H Jane Dyson, Peter E Wright
Jul 18, 2003·Biology of the Cell·Philippe Gonzalo, Jean-Paul Reboud
Jan 1, 1959·Advances in Protein Chemistry·W KAUZMANN

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Citations

Jan 30, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Miaoqing HuAihua Liang
Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Yuichi ShigeokaYoichi Aso
Apr 16, 2013·Journal of Biomolecular Structure & Dynamics·Pushpa MishraRamakrishna V Hosur
Oct 13, 2012·The Journal of Biological Chemistry·Sudipta DasShobhona Sharma
Jul 28, 2020·Biochimie·Pushpa MishraShobhona Sharma

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Methods Mentioned

BETA
GTPase
X-ray
two-hybrid
protein folding
NMR
light scattering
dynamic light scattering
affinity purification

Software Mentioned

PDB2PQR
PYMOL
protscale
ClustalW
ExPASy
Tasser

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