NMR investigations of interactions between anesthetics and lipid bilayers

Biochimica Et Biophysica Acta
Vasco CastroArnold Maliniak

Abstract

Interactions between anesthetics (lidocaine and short chain alcohols) and lipid membranes formed by dimyristoylphosphatidylcholine (DMPC) were studied using NMR spectroscopy. The orientational order of lidocaine was investigated using deuterium NMR on a selectively labelled compound whereas segmental ordering in the lipids was probed by two-dimensional 1H-13C separated local field experiments under magic-angle spinning conditions. In addition, trajectories generated in molecular dynamics (MD) computer simulations were used for interpretation of the experimental results. Separate simulations were carried out with charged and uncharged lidocaine molecules. Reasonable agreement between experimental dipolar interactions and the calculated counterparts was observed. Our results clearly show that charged lidocaine affects significantly the lipid headgroup. In particular the ordering of the lipids is increased accompanied by drastic changes in the orientation of the P-N vector in the choline group.

References

Aug 1, 1977·Quarterly Reviews of Biophysics·J Seelig
Aug 8, 1978·Biochemistry·J Seelig, N Waespe-Sarcevic
Jun 1, 1987·Krankenpflege Journal·D Blumenberg
Feb 1, 1966·Biochemistry·N E GoodR M Singh
Feb 17, 1994·Nature·N P Franks, W R Lieb
Mar 26, 1998·Biochimica Et Biophysica Acta·D P TielemanH J Berendsen
Mar 24, 2000·Journal of Magnetic Resonance·D McElhenyL Frydman
May 16, 2000·Chemistry and Physics of Lipids·I Ueda, T Yoshida
Jul 5, 2001·Colloids and Surfaces. B, Biointerfaces·P T. Frangopol, D Mihăilescu
Aug 3, 2001·Die Naturwissenschaften·B Antkowiak
Nov 1, 2002·Biophysical Chemistry·Leonardo Fernandes FracetoEneida de Paula
Aug 23, 2003·Journal of Magnetic Resonance·Sergey V DvinskikhDick Sandström
Aug 29, 2003·Biophysical Journal·Rainer A BöckmannHelmut Grubmüller
Apr 3, 2004·Biophysical Chemistry·Mario SuwalskyCarlos P Sotomayor
May 14, 2004·Journal of Magnetic Resonance·Sergey V DvinskikhDick Sandström
Dec 6, 2005·Biophysical Journal·Michael PatraMikko Karttunen
Nov 23, 2006·Biophysical Chemistry·Carl-Johan HögbergAlexander P Lyubartsev
Jan 26, 2007·The Journal of Physical Chemistry. B·Magdalene BaciuMarc S Leaver
Jun 19, 2007·Biochimica Et Biophysica Acta·Vasco CastroArnold Maliniak
Aug 28, 2007·Biophysical Journal·Carl-Johan Högberg, Alexander P Lyubartsev
Mar 18, 2008·The Journal of Physical Chemistry. B·Emma TeramaIlpo Vattulainen

❮ Previous
Next ❯

Citations

Nov 3, 2010·Biophysical Chemistry·Enamul H Mojumdar, Alexander P Lyubartsev
Oct 29, 2015·The Journal of Physical Chemistry. B·Alexandru BotanJoona Petteri Tynkkynen
Jul 25, 2013·Journal of Magnetic Resonance·Axel GansmüllerSabine Hediger
Jul 31, 2012·Biochimica Et Biophysica Acta·Nicole WeizenmannHolger A Scheidt
Apr 12, 2017·Journal of Molecular Modeling·Abbas YousefpourSepideh Amjad-Iranagh
Aug 8, 2015·Physical Chemistry Chemical Physics : PCCP·Jon KaplaArnold Maliniak
Apr 27, 2018·Journal of Biomolecular Structure & Dynamics·Gholamhossein Sodeifian, Fariba Razmimanesh
Aug 21, 2012·Physical Chemistry Chemical Physics : PCCP·Mária DarvasPál Jedlovszky
Jun 18, 2019·Biochimica Et Biophysica Acta. General Subjects·Masanao KinoshitaNobuaki Matsumori
Oct 26, 2018·Chemical Reviews·Victoria Oakes, Carmen Domene
Oct 25, 2013·Analytical Chemistry·Da HuangPaul S Cremer
Sep 4, 2021·Molecular Pharmacology·Stephan L GrageStefan Weinschenk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.