NMR metabolomic study of blood plasma in ischemic and ischemically preconditioned rats: an increased level of ketone bodies and decreased content of glycolytic products 24 h after global cerebral ischemia

Journal of Physiology and Biochemistry
Eva BaranovicovaJ Lehotský

Abstract

Cardiac arrest is one of the leading causes of death among adults in older age. Understanding mechanisms how organism responds to ischemia at global level is essential for the prevention and ischemic patient's treatment. In this study, we used a global cerebral ischemia induced by four-vessel occlusion as an established animal model for ischemic stroke to investigate metabolic changes after 24 h reperfusion, when transitions occur due to the onset of delayed neuronal death. We also focused on the endogenous phenomenon known as ischemic tolerance by the pre-ischemic treatment. The experiments were carried out on blood plasma samples as easily available and metabolically reflecting the overall changes in injured organism. Our results imply that disturbed glycolysis pathway, as a consequence of ischemic injury, leads to the increased level of ketone bodies (acetone, acetoacetate and β-hydroxybutyrate) along with increased utilization of triacylglycerols in plasma of ischemic and ischemically preconditioned rats. Complementary to, a decreased level of glycolytic intermediates (lactate, pyruvate, acetate) with increased level of glucose was found in ischemic and preconditioned animals. The protective effect of ischemic preconditioni...Continue Reading

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Citations

Jul 19, 2020·Nature Protocols·Isabel Garcia-PerezJeremy K Nicholson
Mar 21, 2021·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Alireza Salehi MMikael Landén
Jul 3, 2021·Reviews in the Neurosciences·Maria S ChumachenkoSergei V Fedorovich

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Methods Mentioned

BETA
NMR
dissect
PCA

Software Mentioned

Random Forest
PLS
DA
OPLS
chenomx
R caret

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