NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis

FEBS Letters
Helena TossavainenRaili Seppala

Abstract

PrsA is a peptidyl-prolyl isomerase (PPIase) from Bacillus subtilis belonging to the parvulin family of PPIases. It is a membrane bound lipoprotein at the membrane-wall interface, involved in folding of exported proteins. We present the NMR solution structure of the PPIase domain of PrsA, the first from a Gram-positive bacterium. In addition we mapped out the active site with NMR titration experiments. A high degree of conservation with other members of the parvulin family was revealed in the structure and binding site. Interactions with substrate peptides were also characterized by mutated domains revealing that H122 is indispensable for overall correct folding.

References

Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Feb 13, 2001·Journal of Molecular Biology·T TeradaT Uchida
Jun 8, 2002·EMBO Reports·Peter E Shaw
Jun 25, 2002·Journal of Molecular Biology·Isabelle LandrieuGuy Lippens
Feb 8, 2003·Chemistry & Biology·Takafumi UchidaTony Hunter
Aug 24, 2004·Protein Science : a Publication of the Protein Society·Angelika KühleweinGerd Gemmecker
Sep 25, 2004·Journal of Magnetic Resonance·Helena Tossavainen, Perttu Permi
Nov 18, 2004·Biochimica Et Biophysica Acta·Matti SarvasJan Maarten van Dijl

❮ Previous
Next ❯

Citations

Jan 10, 2008·Trends in Microbiology·Colin R Harwood, Rocky Cranenburgh
Oct 30, 2009·Protein Science : a Publication of the Protein Society·Ulrich WeiningerFranz X Schmid
Apr 29, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Björn M Burmann, Sebastian Hiller
Nov 16, 2011·Journal of the American Chemical Society·Jonathan W MuellerWulf Blankenfeldt
Apr 25, 2006·The Journal of Biological Chemistry·Xue KangChangwen Jin
Dec 20, 2014·The Journal of Biological Chemistry·Roman P JakobTimm Maier
Jan 1, 2008·Perspectives in Medicinal Chemistry·Jonathan W Mueller, Peter Bayer

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.