PMID: 11933230Apr 5, 2002Paper

NMR solution structure, backbone mobility, and homology modeling of c-type cytochromes from gram-positive bacteria

Chembiochem : a European Journal of Chemical Biology
L BanciAndrew R Thompsett

Abstract

The solution structure of oxidized cytochrome c(553) (71 amino acid residues) from the Gram-positive bacterium Bacillus pasteurii is here reported and compared with the available crystal structure. The solution structure is obtained from 1609 meaningful NOE data (22.7 per residue), 76 dihedral angles, and 59 pseudocontact shifts. The root mean square deviations from the average structure are 0.25+/-0.07 and 0.59+/-0.13 A for the backbone and all heavy atoms, respectively, and the quality assessment of the structure is satisfactory. The solution structure closely reproduces the fold observed in the crystal structure. The backbone mobility was then investigated through amide (15)N relaxation rate and (15)N-(1)H NOE measurements. The protein is rigid in both the sub-nanosecond and millisecond time scales, probably due to the relatively large heme:number of amino acids ratio. Modeling of eight c-type cytochromes from other Gram-positive bacteria with a high sequence identity (>30 %) to the present cytochrome c(553) was performed. Analysis of consensus features accounts for the relatively low reduction potential as being due to extensive heme hydration and indicates residues 34-35, 44-46, 69-72, and 75 as a conserved hydrophobic pat...Continue Reading

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Citations

Sep 10, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ivano BertiniAntonio Rosato
Sep 11, 2002·Journal of Molecular Biology·Ilaria BartalesiPaola Turano
Mar 4, 2005·Chembiochem : a European Journal of Chemical Biology·Jonathan A R WorrallGerard W Canters
May 12, 2004·Journal of Inorganic Biochemistry·Susan J Berners-PricePaola Turano
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Mar 25, 2011·Metallomics : Integrated Biometal Science·Md Khurshid Alam KhanFaizan Ahmad
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Mar 2, 2011·Metallomics : Integrated Biometal Science·Ivano BertiniAntonio Rosato
Jan 13, 2006·Chemical Reviews·Ivano BertiniAntonio Rosato
Aug 3, 2021·Journal of Biomolecular NMR·Stefano CucuzzaOliver Zerbe
Jan 11, 2022·Chemical Reviews·Thomas MüntenerSebastian Hiller

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