NMR solution structures of LNA (locked nucleic acid) modified quadruplexes.

Nucleic Acids Research
Jakob T NielsenMichael Petersen

Abstract

We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.

References

Apr 18, 1991·Nature·E H Blackburn
Aug 1, 1988·Journal of Biomolecular Structure & Dynamics·R Lavery, H Sklenar
Feb 1, 1989·Journal of Biomolecular Structure & Dynamics·R Lavery, H Sklenar
Jul 15, 1981·Journal of Molecular Biology·R E Dickerson, H R Drew
May 25, 1983·Journal of Molecular Biology·R E Dickerson
Dec 8, 1995·Science·V A Zakian
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R WyattD J Ecker
Dec 20, 1993·Journal of Molecular Biology·Y Wang, D J Patel
Nov 21, 1997·Journal of Molecular Biology·K PhillipsB Luisi
Sep 3, 1999·The Journal of Biological Chemistry·P J BatesD M Miller
Sep 14, 2000·Chemistry : a European Journal·K BondensgaardJ P Jacobsen
Jun 29, 2001·Journal of Molecular Biology·M P Horvath, S C Schultz
Dec 18, 2001·Biopolymers·M A Keniry
May 23, 2002·Journal of the American Chemical Society·Michael PetersenJens Peter Jacobsen
Jun 7, 2002·Nature·Gary N ParkinsonStephen Neidle
Jun 25, 2002·Journal of Molecular Biology·Shozeb HaiderStephen Neidle
Jul 18, 2002·Nature Reviews. Drug Discovery·Stephen Neidle, Gary Parkinson
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Evonne M RezlerLaurence H Hurley
Feb 8, 2003·Trends in Biotechnology·Michael Petersen, Jesper Wengel
Apr 12, 2003·Nucleic Acids Research·Virna DapićPaula J Bates
Jul 2, 2003·Current Opinion in Structural Biology·Stephen Neidle, Gary N Parkinson
Jan 30, 2004·Organic & Biomolecular Chemistry·Jesper Wengel
Apr 22, 2004·Journal of the American Chemical Society·Pamela K Dominick, Michael B Jarstfer
Jun 8, 2004·Nucleic Acids Research·Antonio RandazzoLuciano Mayola
Dec 23, 2004·Biopolymers·Luigi PetracconeConcetta Giancola

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Citations

Feb 9, 2013·Chemical Reviews·Osman DolucaVyacheslav V Filichev
Oct 3, 2006·Nucleic Acids Research·Sarah BurgeStephen Neidle
Sep 5, 2007·Nucleic Acids Research·Isabelle LebarsJean-Jacques Toulmé
Nov 11, 2010·Nucleic Acids Research·Erik B PedersenVyacheslav V Filichev
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Timsi RaoDeborah S Wuttke
Feb 6, 2007·FEBS Letters·Carmelo Di PrimoJean-Jacques Toulmé
Feb 17, 2009·Bioorganic & Medicinal Chemistry·Veronica EspositoAldo Galeone
Feb 1, 2008·ChemMedChem·Tian-miao OuLian-quan Gu
Sep 1, 2006·Biopolymers·Luigi PetracconeConcetta Giancola
Oct 31, 2007·Angewandte Chemie·Niti KumarMichael Petersen
Jan 26, 2011·Chemistry : a European Journal·Devranjan PradhanMichael Petersen
Mar 25, 2011·Angewandte Chemie·Yamuna Krishnan, Friedrich C Simmel
Dec 3, 2014·European Journal of Medicinal Chemistry·Valeria RomanucciGiovanni Di Fabio
Oct 31, 2007·Methods : a Companion to Methods in Enzymology·Mateus Webba da Silva
Mar 27, 2013·Journal of Biomolecular Structure & Dynamics·Janos Sagi
Feb 18, 2014·Acta Crystallographica. Section D, Biological Crystallography·Irene Russo KraussFilomena Sica
Jul 27, 2011·Chemical Society Reviews·Gavin W Collie, Gary N Parkinson
Jul 1, 2017·Journal of Proteome Research·Wilson Wen Bin Goh, Limsoon Wong
Nov 7, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andreas Ioannis Karsisiotis, Mateus Webba da Silva
Dec 5, 2018·Chembiochem : a European Journal of Chemical Biology·Linn HaaseKlaus Weisz
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maja Marušič, Janez Plavec
Sep 19, 2008·American Journal of Physiology. Renal Physiology·Maro OkamuraMasanori Kitamura
May 27, 2016·The Journal of Microbiology·Jae-Min Song
Sep 6, 2020·Nucleic Acids Research·Linn Haase, Klaus Weisz
Oct 5, 2018·Nucleic Acids Research·Hehua LiuJianhua Gan
Feb 2, 2022·Journal of Biomolecular Structure & Dynamics·Rupak PalAnsuman Lahiri

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Methods Mentioned

BETA
X-ray
NMR

Software Mentioned

FELIX
AMBER
TL
TGLGLT
Sparky

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