NMR structure of HMfB from the hyperthermophile, Methanothermus fervidus, confirms that this archaeal protein is a histone

Journal of Molecular Biology
M R StarichM F Summers


The three-dimensional structure of the recombinant histone rHMfB from Methanothermus fervidus, an archaeon that grows optimally at 83 degrees C, has been determined by nuclear magnetic resonance methods. This is only the third structure of a protein from a hyperthermophilic organism (optimal growth at temperatures above 80 degrees C). Signal assignments were made using a combination of homonuclear-correlated, 15N-double resonance and 15N, 13C triple resonance NMR experiments. Long range dipolar interactions for the symmetric homodimer were identified from two-dimensional 13C-double half-filtered and three-dimensional 13C-filtered NMR data obtained for a heterolabeled-dimer. A family of 33 structures was calculated using DSPACE with a total of 609 NOE-derived interproton distance restraints, including 22 intraresidue, 192 sequential, 300 medium-range (two to five residues), 86 long-range intramolecular (more than five residues) and 112 intermolecular distance restraints. The monomer subunits consist of three alpha-helices, extending from residues Pro4 to Ala15 (helix I), Ser21 to Ala50 (helix II) and Lys56 to Lys68 (helix III), as well as two short segments of beta-strand comprised of residues Arg19 to Ser21 and Thr54 to Ile55. ...Continue Reading


Jan 7, 1998·Protein Science : a Publication of the Protein Society·T LazaridisM Karplus
Sep 10, 1998·Nature Structural Biology·Y G GaoA H Wang
Jul 17, 1998·Nature Structural Biology·P AgbackT Härd
Jan 29, 2000·Molecular Microbiology·D MusgraveA Slesarev
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Steven P WilkinsonE Peter Geiduschek
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·W F Doolittle
Aug 30, 2008·The Journal of Biological Chemistry·Andrew J AndrewsKarolin Luger
Jan 1, 1997·Nucleic Acids Research·A D Baxevanis, D Landsman
Apr 15, 1997·Nucleic Acids Research·W A LaMarrP C Dedon
Feb 21, 1998·Nucleic Acids Research·A D Baxevanis, D Landsman
Sep 23, 2000·Nucleic Acids Research·F BologneseR Mantovani
Jul 26, 2000·Molecular Biology and Evolution·G WuM Mllerm
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S D Bell, S P Jackson
Sep 22, 2001·Protein Science : a Publication of the Protein Society·R L FahrnerA Slesarev
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Jerry Eichler, Michael W W Adams
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·V Ramakrishnan
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·S L PereiraJ N Reeve
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·J Zlatanova
May 1, 1996·FEMS Microbiology Reviews·R A GraylingJ N Reeve
Oct 21, 2016·International Journal of Molecular Sciences·Jacek DygutIrena Roterman
Jun 27, 1997·Cell·J N ReeveC J Daniels
Nov 13, 1998·Journal of Molecular Biology·T H TahirovI Kato
Feb 12, 1999·Journal of Molecular Biology·K ZemzoumiR Mantovani
Nov 1, 2002·Molecular Cell·Finn Werner, Robert O J Weinzierl
Mar 5, 1999·Protein Engineering·I N BerezovskyL L Kisselev
Sep 27, 2014·International Journal of Molecular Sciences·Valeria VisoneMaria Ciaramella
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Ganggang WangZihe Rao
Nov 22, 2017·Journal of Biochemistry·Masako Koyama, Hitoshi Kurumizaka
Apr 21, 2004·Protein Science : a Publication of the Protein Society·Douglas D Banks, Lisa M Gloss
May 8, 2018·The FEBS Journal·Sudipta BhattacharyyaKarolin Luger
Oct 2, 1998·The Journal of Experimental Zoology·D G Searcy, S H Lee
Mar 29, 2000·Cell Biochemistry and Biophysics·M Hoppert, F Mayer
Dec 22, 2007·Protein Science : a Publication of the Protein Society·Matthew R Stump, Lisa M Gloss
May 31, 2012·Science China. Life Sciences·ZhenFeng ZhangLi Huang
Mar 26, 1998·Protein Science : a Publication of the Protein Society·R Aurora, G D Rose
Nov 7, 2019·ELife·Maria RojecTobias Warnecke

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.