NMR structure of the flavin domain from soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)

Biochemistry
Lisa L ChatwoodS J Lippard

Abstract

Soluble methane monooxygenase (sMMO) catalyzes the hydroxylation of methane by dioxygen to methanol, the first step in carbon assimilation by methanotrophs. This multicomponent system transfers electrons from NADH through a reductase component to the non-heme diiron center in the hydroxylase where O(2) is activated. The reductase component comprises three distinct domains, a [2Fe-2S] ferredoxin domain along with FAD- and NADH-binding domains. We report the solution structure of the reduced 27.6 kDa FAD- and NADH-binding domains (MMOR-FAD) of the reductase from Methylococcus capsulatus (Bath). The FAD-binding domain consists of a six-stranded antiparallel beta-barrel and one alpha-helix, with the first 10 N-terminal residues unstructured. In the interface between the two domains, the FAD cofactor is tightly bound in an unprecedented extended conformation. The NADH-binding domain consists of a five-stranded parallel beta-sheet with four alpha-helices packing closely around this sheet. MMOR-FAD is structurally homologous to other FAD-containing oxidoreductases, and we expect similar structures for the FAD/NADH-binding domains of reductases that occur in other multicomponent monooxygenases.

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Citations

Dec 8, 2005·Journal of the American Chemical Society·Jessica L BlazykStephen J Lippard
May 20, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Stephen J Lippard
Jun 18, 2014·Journal of the American Chemical Society·Weixue WangStephen J Lippard
Feb 18, 2010·Archives of Biochemistry and Biophysics·Michelle OppenheimerPablo Sobrado
Aug 27, 2015·Biochemistry·Justin F AchesonBrian G Fox
Jul 2, 2016·Journal of the American Chemical Society·Thomas J Lawton, Amy C Rosenzweig
Mar 18, 2016·Microbiology and Molecular Biology Reviews : MMBR·Alan A DiSpiritoStéphane Vuilleumier
Oct 17, 2019·Science Advances·Hanseong KimUhn-Soo Cho
Nov 24, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Matthew O Ross, Amy C Rosenzweig
Jan 12, 2019·Annual Review of Biochemistry·Rahul BanerjeeJohn D Lipscomb
Jan 26, 2021·Chemical Society Reviews·Christopher W Koo, Amy C Rosenzweig
Aug 10, 2006·Chemical Reviews·Stephen W Ragsdale
Aug 16, 2006·Accounts of Chemical Research·Matthew H Sazinsky, Stephen J Lippard
May 9, 2021·Applied and Environmental Microbiology·Philip DershwitzAlan A DiSpirito
Nov 5, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Chaemin LeeSeung Jae Lee

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