NMR studies of BPTI aggregation by using paramagnetic relaxation reagents

Biochimica Et Biophysica Acta
Andrea BerniniNeri Niccolai

Abstract

Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.

References

Feb 20, 1987·Journal of Molecular Biology·E Tüchsen, C Woodward
Mar 1, 1993·Journal of Biomolecular NMR·T SzyperskiK Wüthrich
Mar 1, 1997·Nature Biotechnology·E Liepinsh, G Otting
May 1, 1996·Nature Biotechnology·C Mattos, D Ringe
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jul 27, 2001·Protein Science : a Publication of the Protein Society·N NiccolaiP A Temussi
Sep 8, 2001·The Journal of Biological Chemistry·N NiccolaiP A Temussi
May 29, 2003·Biophysical Journal·Michael GottschalkBertil Halle
Sep 2, 2003·Journal of Molecular Biology·Neri NiccolaiPiero A Temussi

❮ Previous
Next ❯

Citations

Dec 7, 2007·Nucleic Acids Research·Vincenzo VendittiSamuel E Butcher
Mar 6, 2007·Biochemical and Biophysical Research Communications·Vincenzo VendittiNeri Niccolai
Jul 1, 2010·Protein Science : a Publication of the Protein Society·Hsiau-Wei LeeJames H Prestegard
Jul 10, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Henry G HockingTobias Madl
Nov 16, 2010·Journal of Structural Biology·Xu WangJames H Prestegard
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Feb 13, 2020·Frontiers in Molecular Biosciences·Jeffrey A PurslowVincenzo Venditti
Jan 28, 2022·Chemical Reviews·Qing MiaoMarcellus Ubbink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.