PMID: 8944758Nov 1, 1996Paper

NMR studies of cooperativity in the tetrahaem cytochrome c3 from Desulfovibrio vulgaris

European Journal of Biochemistry
D L TurnerA V Xavier

Abstract

The thermodynamic properties of the Desulfovibrio vulgaris (Hildenborough) tetrahaem cytochrome c3 (Dvc3) are rationalised by a model which involves both homotropic (e-/e-) and heterotropic (e-/H+) cooperativity. The paramagnetic shifts of a methyl group from each haem of the Dvc3 have been determined in each stage of oxidation at several pH values by means of two-dimensional exchange NMR. The thermodynamic parameters are obtained by fitting the model to the NMR data and to redox titrations followed by visible spectroscopy. They show significant positive cooperativity between two of the haems whereas the remaining interactions appear to be largely electrostatic in origin. These parameters imply that the protein undergoes a proton-assisted two-electron transfer which can be used for energy transduction. Comparison with the crystal structure together with measurement of the kinetics of proton exchange suggest that the pH dependence is mediated by a charged residue(s) readily acessible to the solvent and close to haem I.

References

Dec 20, 1979·Nature·R HaserJ Le Gall
Dec 15, 1992·European Journal of Biochemistry·D L TurnerA V Xavier
May 1, 1968·Quarterly Reviews of Biophysics·J Wyman
Sep 1, 1982·European Journal of Biochemistry·J J MouraA V Xavier
Jan 5, 1995·Biochimica Et Biophysica Acta·D L TurnerA V Xavier
Aug 30, 1994·Biochimica Et Biophysica Acta·R J Williams
Dec 5, 1993·Journal of Molecular Biology·P M MatiasM A Carrondo
Jan 1, 1993·Methods in Enzymology·A V XavierH Santos

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Citations

Nov 2, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Noriyuki AsakuraIchiro Okura
Aug 30, 2013·Accounts of Chemical Research·Catarina M Paquete, Ricardo O Louro
Nov 9, 2002·European Journal of Biochemistry·Ilídio J CorreiaAntónio V Xavier
Feb 15, 2000·European Journal of Biochemistry·G ChottardM Bruschi
Mar 15, 1997·European Journal of Biochemistry·C A SalgueiroA V Xavier
Sep 12, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ricardo O Louro
Dec 3, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Bruno M FonsecaRicardo O Louro
Aug 2, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Joana M DantasCarlos A Salgueiro
Oct 25, 2011·FEBS Letters·Cláudio M Soares, António M Baptista
Jun 23, 2007·Biochemical and Biophysical Research Communications·Leonor MorgadoCarlos A Salgueiro
Oct 6, 2015·Biochimica Et Biophysica Acta·Mónica N AlvesCatarina M Paquete
Aug 19, 2007·Biochimica Et Biophysica Acta·Catarina M PaqueteTeresa Catarino
Dec 17, 2008·Biochimica Et Biophysica Acta·Miguel PessanhaAntónio V Xavier
Feb 7, 2013·Biochimica Et Biophysica Acta·Pedro O QuintasDavid L Turner
Feb 24, 2007·Biochimica Et Biophysica Acta·Catarina M PaqueteAntónio V Xavier
Jan 24, 2007·FEMS Microbiology Letters·Andreia F VeríssimoMiguel Teixeira
Nov 29, 2005·Journal of Bioscience and Bioengineering·Toshitaka GotoIsao Yumoto
Oct 18, 2005·Journal of Molecular Biology·Laetitia PieulleE Claude Hatchikian
Apr 24, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Telma C SantosCarlos A Salgueiro
Dec 24, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Cláudio M SoaresMiguel Teixeira
Nov 13, 2008·Proteins·Zhong Zheng, M R Gunner
Oct 1, 2004·The Journal of Biological Chemistry·Ilídio J CorreiaAntónio V Xavier
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Pedro M MatiasMaria Arménia Carrondo

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