PMID: 6966158Apr 30, 1980Paper

NMR studies of the interaction of gene-V protein of bacteriophage M13 with oligonucleotides

Biochimica Et Biophysica Acta
G J GarssenC W Hilbers

Abstract

This paper describes the preparation of deuterated phenylalanine ([2H7]-phenylalanine) and the isolation of phage M13 encoded gene-V protein in which this deuterated amino acid was incorporated. Using this protein spectral assignments of resonances in the aromatic region of the 1H-NMR spectrum of the gene-V protein have been made. Furthermore the interaction of the gene-V protein with the tetranucleotide d(pC-G-C-G) and the hexanucleotide d(pC-G-C-G-C-G) was investigated. From the changes in the aromatic region of the NMR spectrum occurring after binding, it is concluded that at least one phenylalanine and one tyrosine is involved in the interaction with the oligonucleotides via stacking.

References

Mar 22, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R J Williams
Jan 1, 1978·Annual Review of Biochemistry·J J Champoux
Jan 1, 1978·Annual Review of Biochemistry·S H Wickner
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G J GarssenC W Hilbers
Jul 12, 1976·Biochemical and Biophysical Research Communications·C HeleneM Yaniv
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P LuW E Daniel
Oct 28, 1976·Biochimica Et Biophysica Acta·D V GriffithsA S Burgen
Dec 1, 1975·CRC Critical Reviews in Microbiology·D T Denhardt
Jan 13, 1976·Biochemistry·E J GabbayW D Wilson
Jul 24, 1974·Biochemical and Biophysical Research Communications·T CuypersW W de Jong
Aug 4, 2010·The Journal of Physical Chemistry. a·Qingsheng WangM Sam Mannan

❮ Previous
Next ❯

Citations

Jan 1, 1982·Progress in Biophysics and Molecular Biology·C Hélène, G Lancelot
Feb 5, 1987·Journal of Molecular Biology·B V Prasad, W Chiu
May 1, 1983·Bioscience Reports·S Bayne, I Rasched
Jan 17, 1984·Biochemistry·G D Brayer, A McPherson
Aug 15, 1991·European Journal of Biochemistry·P J FolkersC W Hilbers
Apr 1, 1987·Journal of Biomolecular Structure & Dynamics·G D Brayer
Sep 15, 1983·Journal of Molecular Biology·G D Brayer, A McPherson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.