NMR studies of zinc binding in a multi-histidinic peptide fragment

Dalton Transactions : an International Journal of Inorganic Chemistry
Maria Antonietta ZorodduRoberto Anedda

Abstract

A multi-histidinic peptide and its minimal models have been investigated for Zn(ii) binding. We have used NMR spectroscopy to probe the binding of zinc to the three repeats (T(1)R(2)S(3)R(4)S(5)H(6)T(7)S(8)E(9)G(10))(3) and to its mono-histidinic minimal models, the 9- and 10-aminoacid fragment. (1)H-(1)H TOCSY, (1)H-(13)C HSQC, (1)H-(1)H NOESY and (1)H-(1)H ROESY multidimensional NMR techniques were performed to understand the details of metal binding sites and the conformational behaviour of the peptides at different pH values and at different ligand to metal molar ratios. Zinc coordination involves imidazole N(delta) of His6 and carboxyl gamma-O of Glu9 residues; interaction with peptide oxygens of the His6-Thr7 or Thr7-Ser8 bonds in a tetrahedral arrangement with the minimal model peptides, cannot be excluded. Zinc coordination involves, at physiologic pH, all the three imidazole N(delta) donors of His6, His16 and His26 as well as carboxyl gamma-O of Glu residues in a tetra, penta or octahedral arrangement with the three repeats, the 30-aminoacid fragment. Zinc complexation induces important structural changes with the C-terminal portion of the ligand, constraining it to leave its disordered conformation. Our results give r...Continue Reading

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Citations

Aug 18, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Caterina MiglioriniHenryk Kozlowski
Sep 26, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Massimiliano PeanaMaria Antonietta Zoroddu
Feb 10, 2016·Colloids and Surfaces. B, Biointerfaces·Kento KiyotakeTakeo Yamaguchi
Nov 5, 2016·Journal of Inorganic Biochemistry·Paulina KołkowskaMagdalena Rowińska-Żyrek
Aug 19, 2015·Journal of Inorganic Biochemistry·Joanna Izabela LachowiczJosefa Maria González-Pérez
Mar 10, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·J I LachowiczD Choquesillo-Lazarte
Feb 18, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Maurizio RemelliMaria Antonietta Zoroddu
Jun 26, 2015·Organic Letters·Suman Kumar MaityAshraf Brik
Dec 4, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Maurizio RemelliMaria Antonietta Zoroddu
Oct 31, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Maria Antonietta ZorodduHenryk Kozlowski
Sep 3, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Valeria M NurchiAndrea Melchior
Sep 18, 2016·Journal of Inorganic Biochemistry·Massimiliano PeanaMaria Antonietta Zoroddu
Nov 14, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Joanna Izabela LachowiczGarth J S Cooper

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