PMID: 3759336Jun 1, 1986Paper

N.m.r. study of conformational changes in lysozyme around the thermal transition point

International Journal of Peptide and Protein Research
M BernardP Jollès

Abstract

Natural abundance carbon-13 n.m.r. at 50.3 MHz has been used to further document the thermal transition that hen egg-white lysozyme undergoes in solution between 20 degrees and 30 degrees. The study focuses on the temperature sensitivity of more than 50 carboxylic, aromatic and aliphatic single carbon resonances for which unambiguous assignments to specific residues are known. The analysis of selective perturbations in chemical shifts indicates that residues located on both edges of the active site cleft and in the hydrophobic box are primarily involved in the temperature-induced conformational transition. N.m.r. results are compared with crystallographic data on low temperature (form A) and high temperature (form B) interconverting lysozyme crystals, taking advantage of the recent availability of quality high resolution maps for B form orthorhombic crystals. In most cases, a good correlation is found at the atomic level between residues involved in the thermal transition in solution and in the crystalline state. Discrete discrepancies are noted for some residues such as Trp-62 and His-15.

References

Mar 28, 1977·Biochimica Et Biophysica Acta·J Saint-BlancardP Jollès
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P J CozzoneP Jollès
Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Mar 29, 1967·Journal of the American Chemical Society·F J Hartdegen, J A Rupley
Mar 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J BerthouP Jollès
Nov 15, 1982·European Journal of Biochemistry·C RedfieldC M Dobson

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Citations

Jul 1, 1990·International Journal of Peptide and Protein Research·M BernardP Jollès
Jan 1, 1989·Free Radical Research Communications·K BobrowskiK L Wierzchowski

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