PMID: 3768321Aug 26, 1986Paper

NMR study of hybrid hemoglobins containing unnatural heme: effect of heme modification on their tertiary and quaternary structures

Biochemistry
K Ishimori, I Morishima

Abstract

The effect of heme modification on the tertiary and quaternary structures of hemoglobins was examined by utilizing the NMR spectra of the reconstituted [mesohemoglobin (mesoHb), deuterohemoglobin (deuteroHb)] and hybrid heme (meso-proto, deutero-proto) hemoglobins (Hbs). The heme peripheral modification resulted in the preferential downfield shift of the proximal histidine N1H signal for the beta subunit, indicating nonequivalence of the structural change induced by the heme modification in the alpha and beta subunits of Hb. In the reconstituted and hybrid heme Hbs, the exchangeable proton resonances due to the intra- and intersubunit hydrogen bonds, which have been used as the oxy and deoxy quaternary structural probes, were shifted by 0.2-0.3 ppm from that of native Hb upon the beta-heme substitution. This suggests that, in the fully deoxygenated form, the quaternary structure of the reconstituted Hbs is in an "imperfect" T state in which the hydrogen bonds located at the subunit interface are slightly distorted by the conformational change of the beta subunit. Moreover, the two heme orientations are found in the alpha subunit of deuteroHb, but not in the beta subunit of deuteroHb, and in both the alpha and beta subunits of m...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G N La MarK C Langry
Jun 25, 1977·Journal of Molecular Biology·D W Seybert, K Moffat
Jun 14, 1976·Journal of Molecular Biology·T AsakuraM F Perutz
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Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Nov 1, 1959·Archives of Biochemistry and Biophysics·A ROSSI-FANELLIA CAPUTO

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Citations

Mar 17, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gurusamy BalakrishnanThomas G Spiro
Jan 24, 2009·Angewandte Chemie·Ljiljana FrukChristof M Niemeyer
Jun 28, 1988·Biochemistry·K Ishimori, I Morishima
Apr 2, 2009·Bioconjugate Chemistry·Maximilian Glettenberg, Christof M Niemeyer

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