NMR study of the peptide present in the principal neutralizing determinant (PND) of HIV-1 envelope glycoprotein gp120

Journal of Biochemical and Biophysical Methods
A V SarmaA C Kunwar

Abstract

The peptide sequence Gly-Pro-Gly-Arg-Ala-Phe (GPGRAF) is present in many principal neutralizing determinants (PND) of the human immunodeficiency virus type-1 (HIV-1). It has been shown that peptides from the PND sequence contain a significant beta turn in the conserved Gly-Pro-Gly-Arg sequence. In order to find out whether or not the smaller subunits also contain this turn, we have studied the NMR of a hexapeptide [GPGPRAF, peptide (I)], a heptapeptide Gly-Pro-Gly-Arg-Ala-Phe-Cys [GPGRAFC, peptide (II)] and a dodecapeptide [GPGRAFGPGRAF, peptide (III)], retaining the side chain protecting groups. Although the majority of conformations for these peptides are disordered, there is a considerable propensity of structures with beta turn in the GPGR sequence. While peptide (I) and peptide (III) seem to have both type I and type II beta turn conformations, peptide (II) shows a propensity of only type II beta turn. The nascent structures obtained in these peptides may get stabilized as the receptor binding conformation in the presence of the receptors, thus playing a significant role in vaccine development against HIV.

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Citations

Aug 15, 2012·Journal of Biomolecular Structure & Dynamics·Alexander M AndrianovAlexander V Tuzikov
Feb 24, 2005·AIDS Research and Human Retroviruses·Oliver HartleyJohn P Moore
Dec 25, 2003·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov, Yuri A Sokolov
Oct 13, 2005·Journal of Biomolecular Structure & Dynamics·A M Andrianov
Aug 20, 2004·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov
Sep 6, 2011·Journal of Chemical Information and Modeling·Alexander M AndrianovAlexander V Tuzikov

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