NMR study of the possible interaction in solution of angiotensin II with a peptide encoded by angiotensin II complementary RNA.

Proceedings of the National Academy of Sciences of the United States of America
H L EatonS F Martin

Abstract

The potential binding of angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) (AII) to a peptide encoded by its complementary RNA (Lys-Gly-Val-Asp-Val-Tyr-Ala-Val) (IIA) has been studied by monitoring the 1H NMR spectrum of IIA in aqueous phosphate or Tris.HCl buffer (2H2O) as it is titrated with AII. For molar ratios of AII/IIA ranging from 0.2 to 1.8, the NMR spectra are unchanged as compared to the spectra of the isolated peptides. Based on these findings, the Kd for the putative biomolecular complex of the two peptides under these conditions is calculated to be greater than 10(-4) M. This result does not support the suggestion of Elton et al. [Elton, T. S., Dion, L.D., Bost, K. L., Oparil, S. & Blalock, J. E. (1988) Proc. Natl. Acad. Sci. USA 85, 2518-2522] that AII and IIA engage in high-affinity binding (Kd approximately 5 x 10(-8) M) with each other.

References

May 15, 1979·Biochemistry·M Liakopoulou-Kyriakides, R E Galardy
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T S EltonJ E Blalock
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Goldstein, D L Brutlag
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L BostJ E Blalock
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R L SofferC Gloff
Jan 20, 1984·Science·E T Kaiser, F J Kézdy
May 31, 1984·Biochemical and Biophysical Research Communications·J E Blalock, E M Smith
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E T Kaiser, F J Kézdy

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Citations

Mar 1, 1992·The International Journal of Biochemistry·R SeyerA Aumelas
Jul 3, 2003·Journal of Bacteriology·Saul G JacchieriRicardo R Brentani
Jul 31, 1990·Biochemical and Biophysical Research Communications·S A WiestM I Steinberg
Mar 12, 1998·Hypertension·P F DillonD D Holsworth
Jun 24, 2020·Journal of Molecular Recognition : JMR·Guy W DayhoffVladimir N Uversky

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