NMR study of the transforming growth factor-alpha (TGF-alpha)-epidermal growth factor receptor complex. Visualization of human TGF-alpha binding determinants through nuclear Overhauser enhancement analysis.

The Journal of Biological Chemistry
C McInnesB D Sykes

Abstract

The study of human transforming growth factor-alpha (TGF-alpha) in complex with the epidermal growth factor (EGF) receptor extracellular domain has been undertaken in order to generate information on the interactions of these molecules. Analysis of 1H NMR transferred nuclear Overhauser enhancement data for titration of the ligand with the receptor has yielded specific data on the residues of the growth factor involved in contact with the larger protein. Significant increases and decreases in nuclear Overhauser enhancement cross-peak intensity occur upon complexation, and interpretation of these changes indicates that residues of the A- and C-loops of TGF-alpha form the major binding interface, while the B-loop provides a structural scaffold for this site. These results corroborate the conclusions from NMR relaxation studies (Hoyt, D. W., Harkins, R. N., Debanne, M. T., O'Connor-McCourt, M., and Sykes, B. D. (1994) Biochemistry 33, 15283-15292), which suggest that the C-terminal residues of the polypeptide are immobilized upon receptor binding, while the N terminus of the molecule retains considerable flexibility, and are consistent with structure-function studies of the TGF-alpha/EGF system indicating a multidomain binding mode...Continue Reading

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Citations

Jan 1, 1997·Biopolymers·C McInnes, B D Sykes
Mar 19, 1999·Protein Science : a Publication of the Protein Society·K J BarnhamR S Norton
Dec 6, 2001·European Journal of Biochemistry·S G ChamberlinD L Turner
May 25, 2002·Journal of Biomolecular Structure & Dynamics·Robert N JorissenAntony W Burgess
Dec 20, 2007·Growth Factors·Brian W Booth, Gilbert H Smith

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