No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Yet Hoi HongGlenn K McConell

Abstract

Nitric oxide (NO) has been shown to be involved in skeletal muscle glucose uptake during contraction/exercise, especially in individuals with Type 2 diabetes (T2D). To examine the potential mechanisms, we examined the effect of local NO synthase (NOS) inhibition on muscle glucose uptake and muscle capillary blood flow during contraction in healthy and T2D rats. T2D was induced in Sprague-Dawley rats using a combined high-fat diet (23% fat wt/wt for 4 wk) and low-dose streptozotocin injections (35 mg/kg). Anesthetized animals had one hindlimb stimulated to contract in situ for 30 min (2 Hz, 0.1 ms, 35 V) with the contralateral hindlimb rested. After 10 min, the NOS inhibitor, N(G)-nitro-l-arginine methyl ester (l-NAME; 5 μM) or saline was continuously infused into the femoral artery of the contracting hindlimb until the end of contraction. Surprisingly, there was no increase in skeletal muscle NOS activity during contraction in either group. Local NOS inhibition had no effect on systemic blood pressure or muscle contraction force, but it did cause a significant attenuation of the increase in femoral artery blood flow in control and T2D rats. However, NOS inhibition did not attenuate the increase in muscle capillary recruitment d...Continue Reading

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Citations

Feb 26, 2016·Free Radical Biology & Medicine·Shaun A MasonGlenn D Wadley
Nov 4, 2016·Nature Reviews. Endocrinology·Lykke SylowThomas E Jensen
Sep 22, 2018·Journal of Applied Physiology·Jarrod P KerrisGlenn K McConell
Oct 28, 2017·Scientific Reports·Dino PremilovacLisa Foa
Mar 24, 2016·American Journal of Physiology. Endocrinology and Metabolism·Yet Hoi HongGlenn K McConell
Aug 23, 2020·Medicine and Science in Sports and Exercise·Andrew C BetikMichelle A Keske
Apr 22, 2018·Nitric Oxide : Biology and Chemistry·Oliver BaumAndreas Zakrzewicz
Oct 11, 2020·Cellular Signalling·Fatmah AlghamdiIan P Salt

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