No effect of the endurance training status on senescence despite reduced inflammation in skeletal muscle of older individuals

American Journal of Physiology. Endocrinology and Metabolism
Estelle BalanLouise Deldicque

Abstract

The aim of the present study was to determine if the training status decreases inflammation, slows down senescence, and preserves telomere health in skeletal muscle in older compared with younger subjects, with a specific focus on satellite cells. Analyses were conducted on skeletal muscle and cultured satellite cells from vastus lateralis biopsies (n = 34) of male volunteers divided into four groups: young sedentary (YS), young trained cyclists (YT), old sedentary (OS), and old trained cyclists (OT). The senescence state and inflammatory profile were evaluated by telomere dysfunction-induced foci (TIF) quantification, senescence-associated β-galactosidase (SA-β-Gal) staining, and quantitative (q)RT-PCR. Independently of the endurance training status, TIF levels (+35%, P < 0.001) and the percentage of SA-β-Gal-positive cells (+30%, P < 0.05) were higher in cultured satellite cells of older compared with younger subjects. p16 (4- to 5-fold) and p21 (2-fold) mRNA levels in skeletal muscle were higher with age but unchanged by the training status. Aging induced higher CD68 mRNA levels in human skeletal muscle (+102%, P = 0.009). Independently of age, both trained groups had lower IL-8 mRNA levels (-70%, P = 0.011) and tended to ha...Continue Reading

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Citations

Nov 7, 2020·The Journal of Clinical Endocrinology and Metabolism·Tamar TchkoniaJames L Kirkland
Jun 24, 2021·Journal of the American Geriatrics Society·Cristina ValenteJoão Espregueira-Mendes
Aug 10, 2021·Experimental Gerontology·Estelle BalanLouise Deldicque

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Methods Mentioned

BETA
biopsy
PCR
biopsies
qFISH

Software Mentioned

ImageJ
qFISH

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