No evidence for basolateral secretion of milk protein in the mammary gland of lactating goats

Journal of Dairy Science
K StelwagenC G Prosser

Abstract

Recent research suggests that a small percentage of milk proteins may be secreted basolaterally, which would have implications for our work on the permeability of tight junctions in the mammary epithelium. In our work, the presence of alpha-lactalbumin (LA) or lactose in plasma is used as an indicator of permeability. The aim of this study was to examine basolateral secretion by determining the presence of milk proteins in efferent mammary lymph. Five Saanen goats were fitted with mammary lymph catheters and were administered intramammary isosmotic bolus infusions of sucrose control solutions or ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid to induce leaky tight junctions. Lymph samples were collected before and approximately 5 h after infusion. Lymph was analyzed by Western blotting for the presence of alpha-casein (CN), beta-CN, and alpha-LA No alpha-CN or beta-CN was detected in lymph, but alpha-LA was detected in all lymph samples. Moreover, the signal was much stronger in samples from goats that were treated with ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, and concentrations of alpha-LA in lymph were significantly increased with this treatment. These changes and the absence...Continue Reading

References

Sep 1, 1992·Research in Veterinary Science·C G ProsserS R Davis
Oct 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T SeddikiM Ollivier-Bousquet
Apr 1, 1985·Journal of Dairy Science·T W Keenan, D P Dylewski
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Dec 1, 1995·Experimental Cell Research·E DevinoyM Ollivier-Bousquet

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Citations

Feb 18, 1999·Journal of Dairy Science·K StelwagenH A McFadden
Jul 1, 1999·Journal of Dairy Science·S J Lacy-HulbertK Stelwagen
Nov 1, 2007·Animal : an International Journal of Animal Bioscience·D PomièsB Rémond
Feb 19, 2013·Animal : an International Journal of Animal Bioscience·M T StumpfP Montagner

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