No evidence for intrathecal IgG synthesis to Epstein Barr virus nuclear antigen-1 in multiple sclerosis

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Naghmeh JafariRogier Q Hintzen

Abstract

Recent studies suggest an intrathecal IgG response against Epstein Barr virus (EBV) in multiple sclerosis (MS), implicating a pathogenic role for the virus in MS. To determine the spectrum of anti-EBV antibodies and B-cell epitopes within EBV nuclear antigen-1 (EBNA-1). Furthermore, to determine whether EBNA-1-specific IgG is produced intrathecally. Immunoblot analysis was used to study the anti-EBV IgG response in serum and cerebral spinal fluid (CSF) in MS and controls. EBNA-1 B-cell epitopes were identified by immunoscreening of 12 residue long peptides, with 11 residue overlap, spanning EBNA-1. Thirteen peptides containing all immunoreactive regions were constructed and used in paired serum and CSF of MS patients (n=17) and controls (n=18). Subsequently, reactivity to the identified immunodominant peptide was analysed in a large cohort of serum and CSF of MS patients (n=114) and disease controls (n=62). No difference was observed in the overall anti-EBV antibody diversity, but EBNA-1 reactivity was increased in MS patients versus controls for immunoblot and ELISA (p<0.0001). Epitope analysis on EBNA-1 revealed one immunodominant region covering residues 394-451: EBNA-1(394-451). Anti-EBNA-1(394-451) IgG levels in serum and ...Continue Reading

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Citations

May 24, 2012·Current Opinion in Rheumatology·Alex Tselis
Dec 17, 2014·Clinical & Translational Immunology·Michael P Pender, Scott R Burrows
Aug 21, 2013·Annals of Neurology·Stephen L HauserJorge R Oksenberg
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Feb 17, 2017·Neurology. Neuroimmunology and Neuroinflammation·Ruth DobsonGavin Giovannoni
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Nov 6, 2020·Expert Review of Clinical Immunology·Klemens Ruprecht
Jun 27, 2021·Journal of Neuroimmunology·Shweta JakhmolaHem Chandra Jha

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