No evidence of significant activity of the multidrug resistance gene product in primary human breast cancer

Annals of Oncology : Official Journal of the European Society for Medical Oncology
S Hegewisch-BeckerD K Hossfeld

Abstract

The discovery of the multidrug resistance (MDR1) gene product P-glycoprotein (P-gp) has been widely seen as an important milestone in our understanding of the mechanisms underlying the clinical phenomenon of the emergence of resistant cells. MDR1 expression has been shown for numerous solid tumors and for virtually all hematologic malignancies. Nevertheless, results regarding MDR1/P-gp expression in human breast cancer have been controversial and the results of clinical trials on modulation of P-gp activity have not been encouraging. MDR1/P-gp expression and the function of the P-gp pump were investigated in 61 tumor samples from patients with primary breast cancers by multiparameter analysis using MDR1-RT-PCR, immunohistochemistry with two MAbs (UIC2 and MRK16) and the rhodamine 123 (Rh123) efflux assay. The cellular composition of the tumor cell suspension was analyzed by using specific MAbs against the P-gp expressing lymphocyte subsets CD4, CD8 and CD56, as well as against the HER-2/neu gene product, which was used to identify breast carcinoma cells. UIC2 and MRK16 revealed a staining positivity in 72% and 75% of samples, respectively. A positive MDR1-RT-PCR signal was detected in 62% of the samples. Nevertheless, no correl...Continue Reading

Citations

Aug 10, 2000·Breast Cancer Research and Treatment·M ArnalS Lizard-Nacol
Dec 14, 2018·Oncoimmunology·Marion BossennecChristine Ménétrier-Caux
May 11, 2001·Japanese Journal of Cancer Research : Gann·A KanzakiY Takebayashi
Jun 8, 2001·International Journal of Cancer. Journal International Du Cancer·I F FaneyteM J van de Vijver
Nov 2, 1999·Cancer Treatment Reviews·N KrögerA R Zander
Jul 2, 2009·Regulatory Toxicology and Pharmacology : RTP·David BrusickWesley Burks

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