No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes

Circulation
Ina GruhUlrich Martin

Abstract

Recent studies have suggested the differentiation of human endothelial progenitor cells (huEPCs) isolated from peripheral blood into cardiomyocytes. This study investigates whether, when cocultured, neonatal rat cardiomyocytes (NRCMs) can induce transdifferentiation of huEPCs into cardiomyocytes. Coculture experiments with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI)-labeled huEPCs and NRCMs have been performed. Cocultures have been analyzed by means of flow cytometry, 3D confocal laser microscopy, species-specific reverse transcriptase-polymerase chain reaction for the expression of human cardiac marker genes, and electron microscopy. Although fluorescence-activated cell sorting (FACS) analysis and conventional wide-field fluorescence microscopy suggested the existence of DiIpos cardiomyocytes in cocultures, no convincing evidence of cardiac differentiation of huEPCs has been obtained. Apparently, DiIpos cardiomyocytes were identified as necrotic NRCMs or NRCM-derived vesicles with high levels of autofluorescence or, alternatively, as NRCMs lying on top of or below labeled huEPCs or huEPC fragments. Accordingly, no expression of human Nkx2.5, GATA-4, or cardiac troponin I was detected. No convincing evidence of...Continue Reading

References

Jan 1, 1994·Mechanisms of Ageing and Development·G GaoK Ollinger
Apr 1, 1996·The International Journal of Biochemistry & Cell Biology·P A Doevendans, M van Bilsen
Jul 21, 1997·The Journal of Experimental Medicine·U MartinA Klos
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·E C SvenssonJ M Leiden
Mar 13, 1999·The Journal of Clinical Investigation·S MakinoS Ogawa
Jan 5, 2000·The Journal of Clinical Investigation·Y LinR P Hebbel
Apr 5, 2001·Nature·D OrlicP Anversa
Aug 30, 2001·The Journal of Biological Chemistry·M JamaliI S Skerjanc
Mar 23, 2002·Circulation Research·Benoit G Bruneau
Jul 2, 2002·The Journal of Thoracic and Cardiovascular Surgery·T KofidisA Haverich
Jan 9, 2003·Lancet·Christof StammGustav Steinhoff
Feb 12, 2004·Cell Proliferation·F D CamargoM A Goodell
Aug 24, 2004·Circulation Research·Carmen Urbich, Stefanie Dimmeler
Oct 19, 2004·Journal of the American College of Cardiology·Volker SchächingerAndreas M Zeiher
Oct 20, 2004·The Journal of Cell Biology·Katsuhisa MatsuuraIssei Komuro

❮ Previous
Next ❯

Citations

Jun 1, 2008·Journal of Cardiovascular Translational Research·Cindy M Martin
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Valentin Fuster, Javier Sanz
Feb 11, 2009·Antioxidants & Redox Signaling·Wolfram-Hubertus Zimmermann
Oct 3, 2006·Cloning and Stem Cells·Peter A HornHeiner Niemann
Jan 1, 2008·Innovations : Technology and Techniques in Cardiothoracic and Vascular Surgery·Kitipan V AromVibul Jotisakulratana
Aug 3, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Qinghua ZengChengwen Sun
Nov 8, 2008·Circulation Research·Hans ReineckeMichael A Laflamme
Jul 17, 2008·Biological Chemistry·Mihail HristovChristian Weber
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano
Jan 1, 2014·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Kamel SadatFadi G Hage
Mar 7, 2014·Cardiology in Review·Robert E Michler
Jan 1, 2014·Advanced Drug Delivery Reviews·Maximilian Y EmmertSimon P Hoerstrup
Jul 12, 2014·Stem Cells and Development·Dario BongiovanniGiulio Pompilio
Jun 9, 2007·Expert Opinion on Biological Therapy·Régis RocheFranck Festy
Jul 8, 2014·Journal of Molecular and Cellular Cardiology·Noemi PavoMariann Gyöngyösi
Apr 17, 2008·Biochemical and Biophysical Research Communications·Kenichi YamaharaKen Suzuki
Apr 12, 2008·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Louis Maximilian Buja, Deborah Vela
Oct 31, 2006·Hearing Research·Shelley A Batts, Yehoash Raphael
Dec 20, 2007·Journal of Cellular and Molecular Medicine·E Y PlotnikovD B Zorov
Aug 10, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Robert E WeliksonStephen D Hauschka
Oct 20, 2010·Cardiovascular Therapeutics·Emerson C Perin, Guilherme V Silva
Jan 15, 2010·Journal of Cellular and Molecular Medicine·Yu Suk ChoiRodney J Dilley
Aug 25, 2007·Journal of Molecular and Cellular Cardiology·Kaomei Guan, Gerd Hasenfuss
Oct 27, 2009·The Annals of Thoracic Surgery·Jeremy L HerrmannDaniel R Meldrum
Jun 7, 2008·Journal of the American College of Cardiology·Stefan RuppStefanie Dimmeler
Mar 20, 2013·BioMed Research International·Calvin C ShengJijun Hao
Aug 15, 2014·PloS One·Constantin RüderDietlind Zohlnhöfer
Apr 3, 2008·Asian Cardiovascular & Thoracic Annals·Kitipan V AromVibul Jotisakulratana
Aug 29, 2009·Asian Cardiovascular & Thoracic Annals·Kitipan V AromMontip Tiensuwan
Aug 24, 2018·Journal of Cardiac Surgery·Robert E Michler
May 5, 2007·Circulation Research·Kaomei GuanGerd Hasenfuss
Mar 15, 2006·Circulation·Philippe Menasché
Jul 16, 2008·Circulation·Christina MauritzUlrich Martin
Jun 10, 2008·The Journal of Pharmacology and Experimental Therapeutics·Qinghua ZengChengwen Sun
Jan 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Alessia OrlandiMaurizio C Capogrossi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.