Abstract
Multipotent mesenchymal stromal cells (MSC) are candidates for cellular therapy in regenerative medicine and as treatment of graft-versus-host-disease (GvHD) after hematopoietic stem cell (HSC) transplantation. It has been suggested that MSC may be trapped in bone marrow (BM) filters during the stem cell procurement and lost from the HSC graft. We investigated filtered BM and filters from six HSC donors. MSC were expanded from the two sources and investigated by flow cytometry, doubling capacity, differentiation ability and suppression in mixed lymphocyte cultures. A range of 0.3-3.4% cells was trapped in the filters. By flow cytometry, there was no difference in the proportions of different cell types between the filter-retrieved and filtered BM cells. The phenotype, immunosuppressive capacity, differentiation and growth were equal in MSC expanded from the two cell sources. Given the low number of trapped cells, filters do not appear to be a good source of MSC. When intended for clinical transplantation, MSC need to be expanded ex vivo to achieve sufficient doses for a clinical effect.
References
Apr 1, 1977·The Journal of Clinical Investigation·R StorbE D Thomas
Jun 1, 1992·Annals of Hematology·Y MoriyamaA Shibata
Jul 1, 1985·Transplantation·O Ringdén, B Nilsson
Mar 4, 1983·JAMA : the Journal of the American Medical Association·M M BortinA A Rimm
Feb 1, 1997·Journal of Cellular Biochemistry·S P BruderS E Haynesworth
Apr 4, 1997·Science·D J Prockop
Apr 2, 1999·Science·M F PittengerD R Marshak
Feb 13, 2001·British Journal of Haematology·A DominiettoA Bacigalupo
Jun 28, 2003·Blood·Norbert C GorinUNKNOWN European Cooperative Group for Blood and Marrow Transplantation Acute Leukemia Working Party
Oct 11, 2003·Experimental Hematology·Katarina Le BlancOlle Ringdén
May 4, 2004·Lancet·Katarina Le BlancOlle Ringdén
Jul 26, 2005·Cytotherapy·K Le Blanc, Mf Pittenger
Sep 6, 2005·Bulletin of Experimental Biology and Medicine·T E ManakovaN D Khoroshko
Oct 21, 2005·Cytotherapy·E M HorwitzUNKNOWN International Society for Cellular Therapy
Apr 11, 2006·Bone Marrow Transplantation·M SundinK Le Blanc
May 30, 2006·Transplantation·Olle RingdénKatarina Le Blanc
Jun 6, 2006·Bone Marrow Transplantation·D VicenteA Bacigalupo
Jan 16, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Koji KatoUNKNOWN Japan Marrow Donor Program
Jan 18, 2007·Journal of Cellular Physiology·Antonio GiordanoIgnazio R Marino
Oct 24, 2007·Journal of Internal Medicine·K Le Blanc, O Ringdén
Citations
Mar 26, 2011·Stem Cells International·Jeffery J AulettaLuis A Solchaga
Aug 21, 2012·Cytotherapy·Dominic ClarkeJean Stanton
Sep 24, 2009·Experimental Hematology·Jaehyup Kim, Peiman Hematti
May 23, 2009·Cytotherapy·Chiara CapelliMartino Introna
Feb 7, 2013·Einstein·Glaziane Cordeiro de DeusLuciana Cavalheiro Marti