PMID: 9192077Jan 1, 1997Paper

No mechanical role for vinculin in strain transduction in primary bovine osteoblasts

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
U MeyerDavid Jones

Abstract

Vinculin is thought to play a central role in linking the actin cytoskeleton to the integrin adhesion proteins of focal contacts and also in cell-cell adhesion sites. We have investigated aspects of attachment and the transduction of mechanical stimulation on the function of vinculin and its assembly in primary bovine osteoblasts. The dynamic attachment process was concomitant with the reassembly of vinculin from a soluble cytoplasmic pool into Triton-insoluble focal adhesions. Immunoblotting experiments demonstrated a slight increase in the amount of vinculin concentrated in focal contacts that was paralleled by a discrete decrease in the Triton-soluble fraction of cytoplasmic vinculin. Sixty cycles of 1 Hz deformations at 0.02% (hypophysiological, no change in cell division rate), 0.2% (within the normal physiological range), and 1% (hyperphysiological) resulted in a rapid and reversible disassembly of vinculin from focal contacts to a homogeneous cytoplasmic localization, although alterations in the shape and morphology of cells could not be detected. Although all mechanical loading protocols dramatically depleted vinculin from focal contacts, its initial distribution in adhesion plaques was fully restored within the next 60...Continue Reading

Citations

Dec 10, 2003·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Hans Peter WiesmannUlrich Meyer
May 18, 2004·International Journal of Oral and Maxillofacial Surgery·U MeyerH P Wiesmann
Apr 29, 2004·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Ulrich MeyerUlrich Joos
Sep 16, 2005·The British Journal of Oral & Maxillofacial Surgery·U MeyerH P Wiesmann

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