PMID: 8957377Dec 1, 1996Paper

No-react detoxification process: a superior anticalcification method for bioprostheses

The Annals of Thoracic Surgery
A AbolhodaS Gabbay

Abstract

Glutaraldehyde pretreatment of bioprosthetic heart valves is the major pathogenic factor in their calcific degeneration. This comparative study investigates the merit of the No-React aldehyde detoxification process as an alternative modifier of xenograft tissues. Glutaraldehyde- and No-React-pretreated porcine aortic valve cusps were implanted subcutaneously in 6-week-old rats (n = 20). At 3, 6, and 14 weeks, randomly selected animals were sacrificed and the explants underwent mineral and morphologic analyses. Glutaraldehyde- and No-React-treated bovine pericardium and porcine aortic valve cusp were incubated in fibroblast cell culture plates. Cell viability was observed under reversed microscope at 6, 24, 48, and 96 hours. Erythrosin B dye exclusion test was used to validate percent cell death. Pretreatment with No-React significantly inhibited calcification of aortic cusp subcutaneous implants throughout the 14-week period (mean tissue Ca2+ content = 1.3 +/- 0.7 micrograms/mg at 14 weeks.) Glutaraldehyde-treated cusps underwent protracted calcification (Ca2+ content = 190.6 +/- 89.5 micrograms/mg; p < 0.01). Morphologic findings correlated with mineral analyses. One-hundred percent of fibroblast cells survived in the presence...Continue Reading

References

Sep 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·D M CosgroveF D Loop

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Citations

Apr 7, 2006·Clinical Research in Cardiology : Official Journal of the German Cardiac Society·Michele MusciR Hetzer
Mar 10, 2001·The Annals of Thoracic Surgery·S M MarianeschiC F Marcelletti
Feb 4, 1999·The Annals of Thoracic Surgery·S OstapczukA Krian
Feb 4, 1999·The Annals of Thoracic Surgery·M DeiwickH H Scheld
Jan 4, 2001·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·R JashariW Flameng
Jun 28, 2011·Journal of Cardiothoracic Surgery·Manuel GaliñanesAndrzej Sosnowski
Apr 1, 2005·The Heart Surgery Forum·Shekar L C ReddyStephen C Clark
Jan 1, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·David C ReinekeLars Englberger
Dec 2, 2004·Expert Opinion on Biological Therapy·Dan T Simionescu
Aug 4, 2015·Interactive Cardiovascular and Thoracic Surgery·Jochen GrohmannMatthias Siepe
May 28, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Ayhan SahinTomas Holubec
Nov 27, 2007·The Annals of Thoracic Surgery·Woong-Han KimChung Il Noh
Feb 4, 2010·Asian Cardiovascular & Thoracic Annals·Andrea ColliTiziano Gherli
Feb 21, 2018·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Shintaro NemotoMasaya Ito
Mar 17, 2020·Journal of Cardiac Surgery·Guglielmo StefanelliLuca Weltert
Dec 11, 2014·Medical Science Monitor Basic Research·Torsten ChristWolfgang Konertz
Jun 20, 2020·The Journal of Cardiovascular Surgery·Florent Le BarsJean-Philippe Verhoye
Jun 1, 2021·Journal of Cardiac Surgery·Arif SelcukNuman A Aydemir

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