PMID: 9435583Jan 22, 1998Paper

NO2-induced generation of extracellular reactive oxygen is mediated by epithelial lining layer antioxidants

The American Journal of Physiology
Leonard W Velsor, E M Postlethwait

Abstract

Nitrogen dioxide (NO2) is an environmental oxidant that causes acute lung injury. Absorption of this aqueous insoluble gas into the epithelial lining fluid (ELF) that covers air space surfaces is, in part, governed by reactions with ELF constituents. Consequently, NO2 absorption is coupled to its chemical elimination and the formation of ELF-derived products. To investigate mechanisms of acute epithelial injury, we developed a model encompassing the spatial arrangements of the lung surface wherein oxidation of cell membranes immobilized below a chemically defined aqueous compartment was assessed after NO2 exposures. Because aqueous-phase unsaturated fatty acids displayed minimal NO2 absorptive activity, these studies focused on glutathione (GSH) and ascorbic acid (AH2) as the primary NO2 absorption substrates. Results demonstrated that membrane oxidation required both gasphase NO2 and aqueous-phase GSH and/or AH2. Membrane oxidation was antioxidant concentration and exposure duration dependent. Furthermore, studies indicated that GSH- and AH2-mediated NO2 absorption lead to the production of the reactive oxygen species (ROS) O-2. and H2O2 but not to .OH and that Fe-O2 complexes likely served as the initiating oxidant. Similar r...Continue Reading

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Citations

Oct 20, 2001·Journal of Applied Physiology·L M ConnorE M Postlethwait
Jan 8, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sadis MatalonErika C Crouch
May 1, 2010·Bulletin of Environmental Contamination and Toxicology·Yingyi ZhangLin Hao
May 19, 2009·The Journal of Physical Chemistry. B·Shinichi EnamiA J Colussi
Jan 15, 2005·Free Radical Biology & Medicine·Carol A BallingerEdward M Postlethwait

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